International Journal of Life Science and Agriculture Research

The utilization of acidic soil is often not maximized due to various problems, especially those related to the physical and chemical properties of the soil. Efforts to increase productivity in acidic soil can be done through the use of biological fertilizers such as Arbuscular Mycorrhizal Fungi (AMF) and Rhizobium, which play a role in increasing phosphorus and nitrogen absorption. This study aims to determine the most effective combination of biological fertilizers for soybean cultivation. The study was conducted in Sirnabaya Village, East Telukjambe District, Karawang Regency, West Java. The experiment was conducted over a period of 3 months, from 

March 2025 to May 2025. The method used was an experimental method using a single-factor Randomized Block Design (RBD). There were a total of 9 treatments repeated 4 times, resulting in 36 experimental units. The treatments consisted of A (control), B (AMF 15 g/plant), C (AMF 20 g/plant), D (Rhizobium 15 ml/kg of seeds), E (AMF 15 g/plant + Rhizobium 15 ml/kg of seeds), F (AMF 20 g/plant + Rhizobium 15 ml/kg seed), G (Rhizobium 20 ml/plant), H (AMF 15 g/plant + Rhizobium 20 ml/kg seed), I (AMF 20 g/plant + Rhizobium 20 ml/kg seed). The observation data were tested using the F test, and if there was a significant effect, it was further tested using the Duncan Multiple Range Test (DMRT) at a 5% level. The results showed that treatment H had the best effect on root infection parameters, root length per plant, root dry weight, number of pods per plant, seed weight per 100 seeds, plant height, and number of leaves. 

Arbuscular Mycorrhizal Fungi, Nutrient uptake, Rhizobia, Soil Microorganism

1. Abdillah, Labib, Mohamad Haris Septian, And Mikael Sihite. 2022. “Potensi Pemanfaatan Mikoriza Arbuskula (Am) Pada Lahan   Hijauan Pakan.” Journal Of Livestock Science And Production 5(2):362–70. Doi: 10.31002/Jalspro.V5i2.5312.     
2. Adi Pratama, Rama, Ahmad Nizar, And Toto Siswancipto. 2019. “Pengaruh Pemberian Berbagai Dosis Cendawan Mikoriza Arbuskular (Cma) Dan Pupuk Fosfat Alam Terhadap Pertumbuhan Dan Hasil Kacang Merah (Phaseolus Vulgaris L.) Lokal Garut.” Agro Wiralodra 2(2):43–51. Doi: 10.31943/Agrowiralodra.V2i2.17. 
3. Dina, Annisa, And Koesriharti Koesriharti Koesriharti. 2022. “Pengaruh Inokulasi Rhizobium Dan Pupuk Anorganik Npk Terhadap Pertumbuhan Dan Hasil Tanaman Kedelai (Glycine Max (L.) Merril).” Produksi Tanaman 010(12):684–93. Doi:10.21776/Ub.Protan.2022.010.12.04.
4. Duan, Hai Xia, Chong Liang Luo, Xia Wang, Ye Sen Cheng, Muhammad Abrar, And Asfa Batool. 2024. “Responses Of Legumes To Rhizobia And Arbuscular Mycorrhizal Fungi Under Abiotic Stresses: A Global Meta-Analysis.” Agronomy 14(11). Doi: 10.3390/Agronomy14112597. 
5. Hapsani, Arie, And Hasan Basri. 2018. “Kajian Peranan Mikoriza Dalam Bidang Pertanian.” Agrica Ekstensia 12(2):74–78. Igiehon, Ozede N., And Olubukola O. Babalola. 2021. “Rhizobium And Mycorrhizal Fungal Species Improved Soybean Yield Under Drought Stress Conditions.” Current Microbiology 78(4):1615–27. Doi: 10.1007/S00284-021-02432-W. 
6. Jumiatun, Jumiatun, Anni Nuraisyah, Novita Tri Anggraini, Eva Rosdiana, Irma Harlianingtyas, And Trismayanti Dwi Puspitasari. 2022. “Respon Pertumbuhan Dan Produksi Kedelai Varietas Anjasmoro Dengan Pemberian Rhizobium Pada Cekaman Kekeringan.” Agropross : National Conference Proceedings Of Agriculture 215–20. Doi:10.25047/Agropross.2022.291.     
7. Kementan (2021). Rencana Strategis (Renstra) Pusat Perpustakaan Dan Penyebaran Teknologi Pertanian (Pustaka) Tahun 2020-2024. Jakarta: Kementerian Pertanian, 2020. 
8. Khan, A, A Muhammad, A A Khan, S Anwar, P A Hollington, Ahmad Khan, Asim Muhammad, Asad Ali Khan, Shazma Anwar, And Philip A Hollington. 2019. “Nitrogen Affects Leaf Expansion And Elongation Rates During Early Growth Stages Of Wheat.” International Journal Of Agriculture & Biology Intl. J. Agric. Biol 21(March):1117–22. Doi: 10.17957/Ijab/15.1001. 
9. Kumara, Danis Mustika, And Rahmad Jumadi. 2022. “Aplikasi Pupuk Hayati Penambat N Terhadap Pertumbuhan Dan Hasil Kedelai Edamame (Glycine Max (L.) Merr.).” Agroplantae: Jurnal Ilmiah Terapan Budidaya Dan Pengelolaan Tanaman Pertanian Dan Perkebunan 11(2):133–43. Doi: 10.51978/Agro.V11i2.466. 
10. L.E. Susilowati1, Syekhfani2. 2014. “J Ournal Of D Egraded And M Ining L Ands M Anagement.” Degraded Andmining Landsmanagement 1(2):535–42. 
11. Lestari, Sherly Meridania, R. Soedradjad, Sigit Soeparjono, And Tri Candra Setiawati. 2019. “Program Studi Agroteknologi, Fakultas Pertanian, Universitas Jember,Jl. Kalimantan 37 Jember. 68121 Program Studi Proteksi Tanaman Fakultas Pertanian, Universitas Jember,Jl. Kalimantan 37 Jember. 68121.” Bioindustri 02(01):319–33. 
12. Liem, Jennifer Larisa, Briliani Ayu Arianita, Shinta Sugiarti, And Yoga Aji Handoko. 2019. “Optimalisasi Bakteri Rhizobium Japonicum Sebagai Penambat Nitrogen Dalam Upaya Peningkatan Produksi Jagung.” Jurnal Galung Tropika 8(1):64. Doi: 10.31850/Jgt.V8i1.413. 
13. Linda Kusumastuti, Agung_Astuti Dan Sarjiyah. 2021. “Kajian Asosiasi Rhizobium Sp.-Mikoriza-Rhizobacteri Indigenous Merapi Terhadap Pertumbuhan Dan Hasil Tiga Varietas Kedelai Di Tanah Pasir Pantai.” (2009):8–15. 
14. Loo, Wan Teng, Kah-Ooi Chua, Purabi Mazumdar, Acga Cheng, And Normaniza Osman. 2022. “Arbuscular Mycorrhizal Symbiosis : A Strategy For Mitigating.” 1–33. 
15. Matondang, Ahmad Mizan, Jumini Jumini, And Syafruddin Syafruddin. 2020. “Pengaruh Jenis Dan Dosis Pupuk Hayati Mikoriza Terhadap Pertumbuhan Dan Hasil Tanaman Cabai (Capsicum Annuum L.) Pada Tanah Andisol Lembah Seulawah Aceh Besar.” Jurnal Ilmiah Mahasiswa Pertanian 5(2):101–10. Doi: 10.17969/Jimfp.V5i2.15025. 
16. Mayasin, Lisa Larastuti S., Hayatiningsih Gubali, And Suyono Dude. 2021. “Growth And Result Analysis Of Two Peanut Varieties (Arachis Hypogaea L.) On Various Dosages Of Vesicular Arbuscular Mycorrhiza.” Jatt 10(2):24–33. 
17. Mpongwana, Sanele, Alen Manyevere, Johnfisher Mupangwa, Conference Thando Mpendulo, And Chuene Victor Mashamaite. 2024. “Improving Soil Fertility Through Dual Inoculation With Arbuscular Mycorrhizal Fungi And Rhizobium On A Eutric Cambisol Cultivated With Forage Legumes In A Semi-Arid Region.” Heliyon 10(2):E24817. Doi: 10.1016/J.Heliyon.2024.E24817.
18. Muzaiyanah, Siti, And Subandi. 2016. “The Role Of Organic Matter For Increasing Soybean And Cassava Production On Dry Soil Acid Land.” Iptek Tanaman Pangan 11(2):149–58. 
19. Nazir, Muhammad, Syakur, And Muyasir. 2017. “Pemetaan Kemasaman Tanah Dan Analisis Kebutuhan Kapur Di Kecamatan Keumala Kabupaten Pidie ( Mapping Soil Acidity And Analysis Of Lime Requirement In District Of Pidie District Keumala ).” Jurnal Ilmiah Mahasiswa Pertanian Unsyiah 2(1):21–30. 
20. Pabela1, Maryam, Hayatiningsih Gubali2*, And Zulzain Ilahude3. 2024. “Pengaruh Inokulasi Rhizobium Dan Pupuk Fosfat Terhadap Pertumbuhan Dan Hasil Tanaman Kacang Tanah (Arachis Hypogea L.).” 13(1):8–16. 
21. Pamungkas, R. Danang S., And Mokhamad Irfan. 2018. “Isolasi Bakteri Rhizobium Dari Tumbuhan Leguminosa Yang Tumbuh Di Lahan Bergambut.” Jurnal Agroteknologi 9(1):31–40. 
22. Parapasan, Yonathan, Adryade Reshi, Gusta Jurusan, Budidaya Tanaman, Perkebunan Politeknik, Negeri Lampung, Jln Soekarno-Hatta, And Rajabasa Bandar Lampung. 2014. “Waktu Dan Cara Aplikasi Cendawan Mikoriza Arbuskular (Cma) Pada Pertumbuhan Bibit Tanaman Kopi The Time And Applications Method Of Vesicular Arbuscular Mycorrhizae (Vam) On Growth Of Coffee Seedlings.” Jurnal Penelitian Pertanian Terapan 13(3):203–8. 
23. Permadi, Karsidi, And Yati Haryati. 2015. “Pemberian Pupuk N, P, Dan K Berdasarkan Pengelolaan Hara Spesifik Lokasi Untuk Meningkatkan Produktivitas Kedelai (Review).” Agrotrop: Journal On Agriculture Science 5(1):1–8. 
24. Permanasari, Indah, Kartika Dewi, Mokhamad Irfan, And Ahmad Taufiq Arminudin. 2016. “Peningkatan Efisiensi Pupuk Fosfat Melalui Aplikasi Mikoriza Pada Kedelai.” Jurnal Agroteknologi 6(2):23. Doi: 10.24014/Ja.V6i2.2237. 
25. Prasetyowati, Kartika, And Yuliani. 2018. “Pengaruh Pemberian Mikroorganisme Lokal ( Mol ), Tricoderma Harzianum , Rhizobium Sp . Dan Kombinasinya Terhadap Pertumbuhan Tanaman Kedelai ( Glycine Max ) Pada Media Tanah Kapur.” Lenterabio 7(3):236–40. 
26. Pulungan, Ahmad Shafwan S. 2013. “Infeksi Fungi Mikoriza Arbuskula Pada Akar Tanaman Tebu (Saccharum Officinarum L).” Jurnal Biosains Unimed 1(1):1–46. 
27. Rahmatullah, Widia. 2018. “Pengaruh Inokulasi Rhizobium Terhadap Penyerapan Cu Dan Pertumbuhan Kacang Tanah ( Arachis Hypogaea L.).” Bioma 13(1):51–59. Doi: 10.21009/Bioma13(1).7. 
28. Rajmi, Saftia Laila, Margarettha, And Refliaty. 2018. “Peningkatan Ketersediaan P Ultisol Dengan Pemberian Fungi Mikoriza Arbuskular.” Journal Agroecotania 1 (2)(2):42–48. 
29. Roni, And Noviantoa Sri Hartatika. 2021. “Dan Sri Hartatik.” 03(02):601–12. Sari, Ramdana, And Retno Prayudyaningsih. 2015. “Rhizobium : Pemanfaatannya Sebagai Bakteri Penambat Nitrogen.” Info Teknis Eboni 12(1):51–64. 
30. Setiawati, M.R, Suryatmana, P, Hindersah, R, Fitriatin, B.N., Herdiyantoro, D. 2014. “Karakterisasi Isolat Bakteri Pelarut Fosfat Untuk Meningkatkan Ketersedian P Pada Media Kultur Cair Tanaman Jagung (Zea Mays L.).” 16(1):30–34. 
31. Shumet, Shemeles Tesfaye, Tewodros Ayalew, Amsalu Gobena Roro, And Hussien Mohammed Beshir. 2022. “Intercropping And Rhizobium Inoculation Affected Microclimate And Performance Of Common Bean (Phaseolus Vulgaris L.) Varieties.” Scientifica 2022. Doi: 10.1155/2022/3471912. 
32. Silitonga, Yusnita Wahyuni, And Muhammad Nizar Hanafiah Nasution. 2020. “Efektivitas Cendawan Mikoriza Arbuskula (Cma) Terhadap Pertumbuhan Dan Produksi Jagung Putih (Zea Mays L.).” Agrium 23(1):36–40. 
33. Simanjuntak, N.K., Missdiani, S. Alby. 2022. “Respon Tanaman Kedelai (Glycine Max (L.) Merril) Terhadap Aplikasi Berbagai Dosis Cendawan Mikoriza Arbuskular (Cma).” Jurnal Ilmu Pertanian Agronitas 4(2):244–52. 
34. Siswanto, Bambang. 2019. “Sebaran Unsur Hara N, P, K Dan Ph Dalam Tanah.” Buana Sains 18(2):109. Doi: 10.33366/Bs.V18i2.1184. 
35. Sitorus, Mariyanti Panduwinata, And Setyono Yudo Tyasmoro. 2021. “Pengaruh Pemberian Inokulan Rhizobium Dan Dosis Pupuk N Terhadap Pertumbuhan Dan Hasil Tanaman Kedelai (Glycine Max L.) The Effect Of Giving Rhizobium Inoculant And N Fertilizer On Growth And Yeild Of Soybean (Glycine Max L.).” Jurnal Produksi Tanaman 9(3):194–203. 
36. Sudiarti, Diah. 2018. “Pengaruh Pemberian Cendawan Mikoriza Arbuskula (Cma) Terhadap Pertumbuhan Kedelai Edamame (Glycin Max).” Jurnal Sainhealth 2(2):5. Doi: 10.51804/Jsh.V2i2.256.5-11. 
37. Sukmawaty, E., And A. Asriani. 2015. “Keragaman Mikoriza Arbuskula Indonesia Dan Perananannya Dalam Ekosistem.” Jurnal Biotek 3(1):45–51.
38. Sulfiah, Siti, Nampiah Sukarno, And Agustin Wydia Gunawan. 2021. “Pembersihan Isi Sel Akar Dan Jenis Warna Tinta Untuk Deteksi Cendawan Mikoriza Arbuskula.” Jurnal Sumberdaya Hayati 7(1):36–40. Doi: 10.29244/Jsdh.7.1.36-40. 
39. Susanti, Ambar, Mazidatul Faizah, And Roni Wibowo. 2018. “Uji Infektifitas Mikoriza Indigenous Terhadap Tanaman Kedelai Terinfeksi Phakopsora Pachyrhizi Syd.” Seminar Nasional Multidisiplin 2018 (September):132–37. 
40. Susanti, Ambar, And Anggi Indah Yuliana. 2024. “Pengaruh Penambahan Dua Pupuk Hayati Terhadap Pertumbuhan Kedelai Pada Tanah Litosol.” Exact Papers In Compilation (Epic) 6(3):48–53. Doi: 10.32764/Epic.V6i3.1233. 
41. Syaiful Anwar, And Rover. 2023. “Analisis Kalium (K) Sebagai Dasar Rekomendasi Pupuk Kcl Untuk Optimalisasi Lahan Perkebunan Kelapa Sawit Dalam Budidaya Tanaman Pangan Di Kecamatan Kuantan Tengah.” Jurnal Agro Indragiri 8(1):11–16. Doi: 10.32520/Jai.V8i1.2454. 
42. Tambanung, Steven, Diane D. Pioh, And Wiesje Kumolontang. 2019. “Analisis Sifat Kimia Tanah Pada Tanah Yang Ditanami Tanman Tomat (Solanum Lycopersicum L.) Di Desa Tonsewer Minahasa.” Cocos 1(2):1–6. 
43. Watoni, Hibbul, Yusnaweti Amir, And Yunita Sabri. 2020. “The Effect Of Multiple Dosages Of Arbuscula Mycorrhizal Fungus (Cma) Inoculan On The Growth Of Pre-Nursery Palm Seeds (Elaeis Guinensis Jacq).” (Cm). 
44. Wen, Zhongling, Minkai Yang, Hongwei Han, Aliya Fazal, Yonghui Liao, Ran Ren, Tongming Yin, Jinliang Qi, Shucun Sun, Guihua Lu, Shuijin Hu, And Yonghua Yang. 2023. “Mycorrhizae Enhance Soybean Plant Growth And Aluminum Stress Tolerance By Shaping The Microbiome Assembly In An Acidic Soil.” Microbiology Spectrum 11(2):1–14. Doi: 10.1128/Spectrum.03310-22. 
45. Widodo, Koko Heru, And Zaenal Kusuma. 2018. “Effects Of Compost On Soul Physical Properties And Growth Of Maize On An Inceptisol.” Jurnal Tanah Dan Sumberdaya Lahan 5(2):959–67. 
46. Yuliyati, Rohanis, Ida Bagus Komang Mahardika, And Anak Agung Sagung Putri Risa Andriani. 2023. “Pengaruh Pemberian Konsentrasi Cuka Kayu Dan Pupuk Hayati Mikoriza Arbuskula Terhadap Pertumbuhan Dan Hasil Tanaman Kedelai Edamame (Glycine Max L. Merrill).” Gema Agro 28(2):92–100. 
47. Yusran, Yusran, Hawalina Hawalina, Hastuti Hastuti, N. Humaerah, Bunga Elim Somba, And I. K. Utami. I. 2022. “Pengujian Kualitas Benih Kedelai Pada Pemberian Inokulasi Rhizobium Sp Dengan Berbagai Tingkat Ketersediaan Air.” Agroland: Jurnal Ilmu-Ilmu Pertanian 29(1):85–96. Doi: 10.22487/Agrolandnasional.V29i1.1216.