International Journal of Life Science and Agriculture Research

The investigation of alternative antimicrobial agents has been increased by the emergence of antibiotic-resistant bacteria. Although essential oils (EOs) are recognized for their broad-spectrum antibacterial properties, their hydrophobicity and instability limit their application. This study examines the potential of nanoemulsion formulations to improve the antibacterial properties of rosemary essential oil. A green method was employed to synthesize silver nanoparticles, which included rosemary oil as a reducing agent. The disk diffusion method was employed to assess the antibacterial activity of the resulting formulation against Pseudomonas aeruginosa and Staphylococcus aureus at varying concentrations. S. aureus exhibited a greater sensitivity than P. aeruginosa, which is likely due to differences in their cell wall structures, as evidenced by the dose-dependent inhibition. These results illustrate the significance of nanoemulsified essential oils in the development of innovative antimicrobial strategies and emphasize their potential as effective antibacterial agents, particularly against Gram-positive bacteria.

Nanotechnology, Essential oils, Pseudomonas aeruginosa, Staphylococcus aureus

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