Antioxidant Activity Test Combination Sarang Semut Extract (Myrmecodia Pendans) And Rosella Flower Extract (Hibiscus Sabdariffa L.) With Dpph (1,1-Difenil-2-Pikrilhidrazil) Method
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DOI:
https://doi.org/10.55677/ijlsar/V01I04Y2022-01Abstract
Antioxidants are compounds that can inhibit oxidation by free radicals by reacting with free radicals to form more stable compounds and are less reactive. Sarang semut (Mymercodia pendans) and rosella flower (Hibiscus sabdariffa L.) contain several active ingredients that can act as antioxidants. The purpose of this research was to determine the antioxidant activity combination of sarang semut extract with rosella flower extract against free radical DPPH (1,1-diphenyl-2-picrylhidrazyl). Withdrawal of active compounds from the sarang semut is done by the extraction process soxhletasi method using ethanol solvent. While the withdrawal of active compounds from rosella flowers carried by the digestion method using solvent of water. The combination of sarang semut extract and rosella flower extract is made in a variety of comparison namely 2:0, 2:1, 2:2, 1:2, and 0:2 are made with a concentration of 0.01%, 0.02%, 0.03% ; 0.04% and 0.05%. Then tested for antioxidant activity by DPPH method is UV-Vis spectrophotometry. From the absorbance data was calculated percent antioxidant activity of control expressed by EC50 ANAVA then tested statistically to determine differences antioxidant activity. From the results showed that the EC50 value in each comparison as follows: 2:0 = 5.4078 mg/ml; 2:1 = 3.2189 mg/ml; 2:2 = 2.7017 mg/ml; 1:2 = 3.3464 mg/ml and 0:2 = 7.5439 mg/ml. So it can be concluded that the combination of sarang semut extract with rosella flower extract at a ratio of 2:2 has the greatest antioxidant activity. The test results showed the price F ANAVA count (202.221)> F table (2.87). At α (0.05) which means that there are significant differences between the antioxidant activity of sarang semut extract, rosella flower extract and the combination of sarang semut extract with rosella flowers extract.
Key words: sarang semut, rosella flowers, antioxidant activity, DPPH, EC50
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