Antibacterial Activity of Pineapple (Ananas comosus) Fruit Peel Extract Against Escherichia coli
DOI:
https://doi.org/10.55677/ijlsar/V03I5Y2024-12Keywords:
Ananas comosus, Antibacterial, Escherichia coli, Peel ExtractAbstract
Escherichia coli is generally considered a harmless bacterium, but numerous strains are well-known for causing diseases globally and frequently exhibit antibiotic resistance. Therefore, it's crucial to discover new natural products to combat these bacteria. Meanwhile, a significant amount of pineapple (Ananas comosus) peel ends up as waste. This study aims to examine the antibacterial activity of pineapple (Ananas comosus) fruit peel extract against E. coli. The preparation of pineapple (Ananas comosus) peel extract involves grinding, maceration with 96% ethanol, and evaporation. The phytochemical test was conducted to assess the presence of active compounds. Flavonoids were identified using Shinoda's test, tannins with Ferric Chloride, and saponins by detecting foaming development. Antibiotic analysis was carried out following the Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. Disks soaked in Ananas comosus peel extract at concentrations of 25%, 50%, 75%, and 100% were placed on Mueller-Hinton (MH) agar inoculated with an E. coli suspension. One-way ANOVA was employed to analyze differences between treatments. The phytochemical test results revealed the presence of flavonoids, tannins, and saponins in pineapple peel extract. The highest inhibition zone, measuring 2.231 mm, was observed with 100% Ananas comosus peel extract. Statistical analysis confirmed significant differences between the groups with a significance value of 0.000 (p<0.05). This suggests that pineapple peel extract could be useful in removing E. coli, rather than being discarded as waste. Further research is needed to gain a better understanding of the antibacterial mechanism.
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