International Journal of Life Science and Agriculture Research

Investigation on the biochemical changes of underlying mechanisms regulating fruit ripening process in Pyrus pashia , a wild edible fruiting species from Rudraprayag , Uttarakhand, was carried out. The fruits were collected in  different maturity stages;  unripe, semi-ripe, and fully ripe and analysed for their morphological and biochemical parameters, flavonoid and phenol contents using  standard AOAC methods. It was found that the fruit skin color and specific gravity values can be used to differentiate the maturity stages. Ripe fruits were found to be nutritionally rich , but the unripe fruits had more of medicinal rich properties. This study shall help to provide information about correct harvesting and post-harvest storage period of the fruits required for optimizing their nutritional value, enhancing shelf life, and exploring their potential for value-added products. It will also promote commercial utilization of this underutilized fruit species, benefiting local communities and fostering sustainable livelihood opportunities.

Ripening, Maturity, Nutritional, Phytochemical, Post-harvest, Storage

1. AOAC, (2002). Association of Official Analytical Chemists, 17 th edition, Washington, DC, USA.
2. Blakey, R., Tesfay, S., and Bertling, I. (2012). Changes in sugars, total protein, and oil in 'Hass' avocado (Persea americana Mill.) fruit during ripening. Journal of Horticultural Science & Biotechnology. 87:381-387.
3. Blakey, R., Tesfay, S., and Bertling, I. (2014). Ripening physiology and quality of 'Hass' avocado (Persea americana Mill.) after cold storage at 1ºC. The Journal of Horticultural Science and Biotechnology. 89: 655-662. 
4. Chandra, E. and Bharati, P. (2020). Physical and nutritional properties of jackfruit at different stages of maturity. The Pharma Innovation Journal.  9(12): 354-357.
5. Dai, J., and Mumper, R. J. (2010). Plant Phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10): 7313-7352. 
6. Elhadi, M.Y, Armando, C.L, Luis, A, Bello-Perez. (2019). Postharvest Physiology and Biochemistry of fruits and vegetables. In Carbohydrates (Eds Elhadi M. Yahia,) Wood Head Publishing Elsevier. Pp 175-205.
7. Fitriningrum, R., Sugiyarto, S. and Susilowati, A. (2013). Analisis kandungan karbohidrat pada berbagai tingkat kematangan buah karika (Carica pubescens) di Kejajar dan Sembungan, Dataran Tinggi Dieng, Jawa Tengah. Bioteknologi 10: 6-14. 
8. Ghasemzadeh, A. and Ghasemzadeh, N. (2011). Flavonoids and phenolic acids: Role and biochemical activity in plants and human . Journal of Medicinal Plants Research. 5(31): 6697-6703.
9. Giribaldi, M., Perugini, I., Sauvage, F.X. and Schubert, A. (2007). Analysis of protein changes during grape berry ripening by 2-DE and MALDI-TOF. Proteomics. 7(17) : 3154-70. 
10. Haq, N. (2006). Jackfruit: Artocarpus heterophyllus, Southampton Center for underutilized crops, University of Southampton, Southampton . 10:50-100. 
11. Hortwitz, W. (1960). Official and Tentative methods of Analysis, Association of the Official Agriculture Chemist, Washington, DC, USA.
12. Jagdeesh, S.L., Reddy, B.S., Basavraj, N., Swamy, G.S.K. and Hegde, L. (2010). Variability studies in physico-chemical qualities of jackfruit (Artocarpus heterophyllus Lam.) of coastal zone of Karnataka. Karnataka Journal of Agricultural Sciences. 23(2):293-297.
13. Kapoor, L., Sinkin, A.J., Doss, C.G.P. and Siva, R. (2022). Fruit ripening : dynamics and integrated analysis of carotenoids and anthocyanins. BMC plant Biol. 11: 22-27. 
14. Marc, A.R., Mureșan, C. C., Pop, A., Marțiș, S.G., Mureșan, E.A., Postolache, N.A. and Rațu, N.R. (2024). An Overview of Ripening Processes. In Ebook, New Discoveries in Ripening process (Eds. R., Alina Marc, C., Carmen Mureșan, C. and A., Elena Mureșan), Intechopen.
15. Markham, K. R. (1982). Techniques of flavonoid identification. pp. 113.
16. Mazumdar , B.C. and Majumdar, K. (2003). Methods on Physicochemical Analysis of Fruits, University College of Agriculture, Calcutta University.
17. Panzanaro, S. (2010). Biochemical characterization of a lipase from olive fruit (Olea europaea L.) Plant Physiology and Biochemistry. 48(9): 741-745.
18. Paul, V. and Pandey, R. (2014). Role of internal atmosphere on fruit ripening and storability-a review. J Food Sci Technol. 51(7):1223-50. 
19. Peñarrieta, J.M., Alvarado, J.A., Bergenståhl, B., and Åkesson, B. (2007). Spectrophotometric methods for the measurement of total phenolic compounds and total flavonoids in Foods. Revista Boliviana de Quimica, 24: 5-9.
20. Pico, J., Gerbrandt, E.M.  and Castellarina. S.D. (2022). Optimization and validation of a SPME-GC/MS method for the determination of volatile compounds, including enantiomeric analysis, in northern highbush blueberries (Vaccinium corymbosum L.). Food Chemistry. 368, 130812.
21. Prasanna, V., Prabha, T.N., Tharanathan, R.N. (2007). Fruit ripening phenomena--an overview. Crit Rev Food Sci Nutr. 47(1):1-19. 
22. Rawat, D., Dash, S.S.,  Sinha, B.K. and Kumar, V.  Banerjee, A. and  Singh, P. (2018). Community structure and regeneration status of tree species in Eastern Himalaya: A case study from Neora Valley National Park, West Bengal, India. Taiwania. 63. 16-24.
23. Sadasivam, S. and Balasubraminan, T. (1987).  In : Practical manual in Biochemistry. Tamil Nadu Agricultural University Coimbatore. pp. p14.
24. Shahidi, F., and Naczk, M. (2003). Phenolics in Food and Nutraceuticals (2nd ed.). CRC Press. 
25. Staveckienė, J., Brigita, M., Elvyra, J. and Jurgita, K. (2024). Effects of different ripening stages on the content of the mineral elements and vitamin C of the fruit extracts of Solanum Species: S. melanocerasum, S. nigrum, S. villosum, and S. retroflexum". Plants . 13( 3): 343.
26. Tlili, N., Tir, M., Benlajnef, H., Khemiri, S., Mejri, H., Rejeb, S. and Khaldi, A. (2014). Variation in protein and oil content and fatty acid composition of Rhus tripartitum fruits collected at different maturity stages in different locations. Ind. Crops Prod.  59:197–201. 
27. Zhang, L.J., Xiao, G.S.,   Yu, Y.S.,  Xu, Y.S.,  Wu, J.J.,  Zou, B. and  Li, L. (2022). Low-oxygen pulping combined with high hydrostatic pressure improve the stability of blueberry pulp anthocyanins and color during storage.Food Control, 138 :108991.