Weed Community Shift and Crop Tolerance in No-Tillage Rice under Sequential Application of Glyphosate and Cyhalofop-butyl plus Ethoxysulfuron
Abstract:
The reliance on chemical weed control in No-Tillage (NT) rice systems often leads to ecological changes in weed community composition and raises concerns regarding crop safety. This research aimed to evaluate the dynamics of weed community shift (SDR) and assess crop tolerance following the sequential application of glyphosate (pre-plant) and a mixture of cyhalofop-butyl + ethoxysulfuron (post-emergence). The experiment was conducted from May to October 2025 at the Experimental Farm of Universitas Padjadjaran, using a Split-Plot Design with three replications. The results indicated a significant weed composition shift from a community dominated by broadleaves (Limnocharis flava and Salvinia molesta) before application to a grass-dominated community (Echinochloa crus-galli and Leptochloa chinensis) at 3 weeks after application (WAA). Regarding crop tolerance, transient phytotoxicity symptoms in the form of leaf chlorosis were observed at 1 WAA, particularly in the highest dose treatment (1.75 L/ha). However, the rice plants demonstrated a rapid physiological recovery mechanism, with symptoms disappearing completely by 3 WAA. This recovery was confirmed by the vegetative growth response, where the number of vegetative tillers showed no significant inhibition compared to the manual weeding control during the active tillering phase. These findings suggest that sequential application of these herbicides effectively manages the weed spectrum shift and remains agronomically safe due to the crop's high resilience.
KeyWords:
Cyhalofop-butyl, Ecological Shift, Ethoxysulfuron, Phytotoxicity, Weeds.
References:
- Beckie, H. J. (2020). Herbicide resistance in plants. Plants, 9(4), 435. https://doi.org/10.3390/plants9040435
- Beckie, H. J., & Harker, K. N. (2017). Our top 10 herbicide-resistant weed management practices. Pest Management Science, 73(6), 1045–1052. https://doi.org/10.1002/ps.4543
- Benbrook, C. M. (2016). Trends in glyphosate herbicide use in the United States and globally. Environmental Sciences Europe, 28, 3. https://doi.org/10.1186/s12302-016-0070-0
- Duke, S. O. (2020). Glyphosate: Environmental fate and impact. Weed Science.
- Kanissery, R., Gairhe, B., Kadyampakeni, D., Batuman, O., & Alferez, F. (2019). Glyphosate: Its environmental persistence and impact on crop health and nutrition. Plants, 8(11), 499. https://doi.org/10.3390/plants8110499
- Khippal, A., Singh, J., & Chhokar, R. S. (2025). Control of complex weed flora in direct seeded rice using bispyribac-sodium in combination with other herbicides. Journal of Cereal Research, 11(3).
- Kumar, S., Shivani, S., Mishra, J. S., Kumar, S., Kumar, U., & Bharati, R. C. (2018). Efficacy of pre- and post-emergence herbicides on complex weed flora in direct-seeded rice (Oryza sativa) in the eastern plains. The Indian Journal of Agricultural Sciences, 88(3), 387–392.
- MacLaren, C., Storkey, J., Menegat, A., Metcalfe, H., & Dehnen-Schmutz, K. (2020). An ecological future for weed science to sustain crop production and the environment: A review. Agronomy for Sustainable Development, 40, 24. https://doi.org/10.1007/s13593-020-00631-6
- Nohatto, M. A., Agostinetto, D., Langaro, A. C., de Oliveira, C., & Ruchel, Q. (2016). Antioxidant activity of rice plants sprayed with herbicides. Pesquisa Agropecuária Tropical, 46(1), 28–34.
- Rifa’i, T., Susanto, H., Nurmauli, N., & Pujisiswanto, H. (2023). Efikasi herbisida isopropil amina glifosat 480 g/l terhadap gulma pada persiapan lahan sawah dengan sistem tanpa olah tanah dan pengaruhnya terhadap tanaman padi. Jurnal Agrotropika, 22(2), 108–118.
- Rouse, C. E., Roma-Burgos, N., Norsworthy, J. K., Tseng, T.-M., Starkey, C. E., & Scott, R. C. (2018). Echinochloa resistance to herbicides continues to increase in Arkansas rice fields. Weed Technology, 32(1), 34–44. https://doi.org/10.1017/wet.2017.82
- Shekhawat, K., Rathore, S. S., & Chauhan, B. S. (2020). Weed management in dry direct-seeded rice: A review on challenges and opportunities for sustainable rice production. Agronomy, 10(9), 1264. https://doi.org/10.3390/agronomy10091264
- Sims, B., Corsi, S., Gbehounou, G., Kienzle, J., Taguchi, M., & Friedrich, T. (2018). Sustainable weed management for conservation agriculture: Options for smallholder farmers. Agriculture, 8(8), 118. https://doi.org/10.3390/agriculture8080118
- Thyssen, G. N., Naoumkina, M., McCarty, J. C., Jenkins, J. N., Florane, C., Li, P., & Fang, D. D. (2018). The P450 gene CYP749A16 is required for tolerance to the sulfonylurea herbicide trifloxysulfuron sodium in cotton (Gossypium hirsutum L.). BMC Plant Biology, 18, 186.
- Umiyati, U., Kurniadie, D., & Deden. (2021). Efektivitas cyhalofop-butyl mengendalikan gulma pada budidaya padi sawah tabela. Agrotechnology Research Journal, 5(2), 125–130. https://doi.org/10.20961/agrotechresj.v5i2.54578
- Yadav, D. B., Yadav, A., & Punia, S. S. (2019). Effectiveness of triafamone + ethoxysulfuron (pre-mix) against complex weed flora in transplanted rice and its residual effects on wheat. Indian Journal of Weed Science, 51(2), 106–110. https://doi.org/10.5958/0974-8164.2019.00025.X
- Zahan, T., Hossain, M. F., Chowdhury, A. K., Ali, M. O., Ali, M. A., Dessoky, E. S., Hassan, M. M., Maitra, S., & Hossain, A. (2021). Herbicide in weed management of wheat (Triticum aestivum L.) and rainy season rice (Oryza sativa L.) under conservation agricultural system. Agronomy, 11(9), 1704.