International Journal of Life Science and Agriculture Research

Background: Aquaculture, particularly shrimp farming, has become an important economic sector in Viet Nam. The microalga Thalassiosira weissflogii is a valuable natural feed due to its high nutritional value and benefits for larval growth and water quality. Optimizing the culture medium is essential for enhancing biomass productivity and nutritional quality of this diatom for aquaculture applications.

Objectives: This study aimed to identify the optimal nutrient medium for maximizing growth, biomass yield, and nutritional quality of the diatom Thalassiosira weissflogii. The comparative evaluation of different nutrient media was undertaken to provide a scientific basis for recommending a suitable medium for large-scale microalgal biomass production in aquaculture.

Methods: The experiment involved culturing Thalassiosira weissflogii in four different nutrient media—AGP, Walne, F/2, and TT3—over an 8-day period, with three replicates per treatment. After cultivation, the algal biomass was analyzed for key nutritional components, including carbohydrates, carotenoids, and silica, to assess the effects of different nutrient media on algal nutritional quality.

Results: The results revealed statistically significant differences among treatments (p < 0.05). The AGP medium exhibited superior performance, achieving the highest growth rate, maximum cell density (30 × 108 cells/mL on day 7), and the greatest mean biomass yield (3.21 ± 0.41 g/L). In terms of nutritional composition, AGP also produced the highest levels of carbohydrates (2.4 mg/0.1 g algae), carotenoids (0.68 µg/L), and silica (22.2%) compared with the other treatments. The Walne, F/2, and TT3 media resulted in lower growth performance and nutrient accumulation.

Conclusion: The study demonstrates that the AGP medium is the optimal choice for culturing Thalassiosira weissflogii, meeting both high biomass productivity and superior nutritional quality requirements. This medium is therefore well suited for the production of natural live feed for seed production and commercial aquaculture.

Biomass, Cell density, Nutrient media, AGP, Walne, TT3, f/2 medium, Thalassiosira weissflogii

1. Abdelhay, R. A., El-Mor, M. S., Salem, M. A. M., Al-Sagheer, A. A., Abd-Elhakim, Y. M., Hassan, B. A., & Mounes, H. A. M. (2025). Effect of nitrogen sources on diatoms growth and nutritional value for enhancing Litopenaeus vannamei larval performance. Animals, 15(4), 466.
2. Anh, H. T. L., Mai, D. T. N., Thu, N. T. H., & Hồng, Đ. Đ. (2010). Isolation of a DHA- and carotenoid-rich heterotrophic marine microalgal strain of the genus Thraustochytrium from the Thi Nai mangrove lagoon, Binh Dinh. Journal of Biotechnology, 8(3A), 459–465.
3. Becker, E. W. (2013). Microalgae: Biotechnology and microbiology. Cambridge University Press, 293pp.
4. Braga, C. B., Junior, J. A., & Ferreira, L. S. (2020). Carotenoid accumulation in marine diatoms under nutrient enrichment. Algal Research, 64, 102702.
5. Brzezinski, M. A. (1985). The Si:C:N ratio of marine diatoms: interspecific variability and the effect of some environmental variables. Journal of Phycology, 21(3), 347–357.
6. Chu, W. L., Lim, Y. W., & Radhakrishnan, A. K. (2019). Nutritional improvement of microalgae cultivation for food and feed applications. Journal of Applied Phycology, 31(2), 1205–1216.
7. Công, N. V., & Dương, N. K. (2014). Effects of AGP culture medium, initial cell density, salinity, and light intensity on the growth of the microalga Thalassiosira weissflogii and experimental biomass production. Can Tho University Journal of Science, (1), 209–217.
8. Công, N. V., Chanakul, P., & cộng sự (2012). Techniques for culturing the biomass of the microalga Thalassiosira weissflogii under experimental conditions. Journal of Science (HCMUAF), 41(4A), 24–32.
9. Công, N. V., Tâm, L. T., Thơm, L. T., Hà, N. C., Hằng, N. T. M., Minh, C. V., Viên, Đ. T. H., & Hồng, Đ. Đ. (2016). Application of the microalga Thalassiosira weissflogii in the production of disease-free shrimp seed. Vietnam Fisheries Magazine, 9, 65–71.
10. Fábregas, J., Abalde, J., Herrero, C., Cabezas, B., & Veiga, M. (1989). Biomass production and biochemical variability of Isochrysis galbana in relation to culture medium. Aquaculture, 78(1–2), 83–96.
11. FAO (2020). Live feeds in marine aquaculture. Food and Agriculture Organization of the United Nations.
12. Fernandes, L. F. (2019). Effect of diatom Thalassiosira weissflogii on ammonia and nitrate absorption in aquaculture systems. Aquaculture Reports, 14, 22–30.
13. Finkel, Z. V., Beardall, J., Flynn, K. J., Quigg, A., Rees, T. A. V., & Raven, J. A. (2009). Phytoplankton in a changing world: Cell size and elemental stoichiometry. Journal of Plankton Research, 32(1), 119–137.
14. Guillard, R. R. L., & Ryther, J. H. (1962). Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Canadian Journal of Microbiology, 8(2), 229–239.
15. Lobus, N. V., Mikhailov, I. S., Kustov, V. N., & Shchuka, S. A. (2024). Multi-element composition of diatom Chaetoceros spp. Marine Biology Research, 20(1), 45–58.
16. López, A., Morales, C., & Pérez, J. (2020). Effect of nitrogen limitation on carotenoid production in diatoms. Journal of Applied Phycology, 32(3), 2123–2134.
17. Madyod, S., Wuthisuthimethavee, S., Pedpradab, P., Khaochamnan, R., Pholmai, S., & Prombanchong, T. (2025). Biochemical composition, β-glucan and phenolic content of a marine diatom Chaetoceros muelleri cultivated in Guillard’s modified medium. PeerJ, 13, e20098.
18. Matos, A. P., Feller, R., Moecke, E. H. S., de Oliveira, J. V., & Derner, R. B. (2019). Chemical composition and carotenoid content of diatoms cultivated under different nutrient regimes. Bioresource Technology, 275, 205–212.
19. Minh, N. V., Tuyền, N. T. T., et al. (2019). Study on the growth and development characteristics of the diatom Thalassiosira sp. under artificial culture conditions. Can Tho University Journal of Science, 54(2), 101–109.
20. Mischler, J. A., Biggs, T. W., & Scott, K. M. (2014). Photosynthetic pigment production in microalgae under nutrient-rich and nutrient-limited conditions. Journal of Phycology, 50(1), 114–123.
21. Nelson, D. M., Tréguer, P., Brzezinski, M. A., Leynaert, A., & Quéguiner, B. (1995). Production and dissolution of biogenic silica in the ocean. Reviews of Geophysics, 33(4), 63–128.
22. Paliwal, C., Panwar, P., Gujar, A., & Shukla, P. (2017). Regulation of carotenoid biosynthesis in microalgae under nutrient stress. Plant Physiology and Biochemistry, 112, 29–37.
23. Pratoomyot, J., Srivilas, P., & Noiraksar, T. (2005). Fatty acids composition of 10 microalgal species. Songklanakarin Journal of Science and Technology, 27(6), 1179–1187.
24. Qi, Y., Wang, X., & Cheng, J. J. (2016). Preparation and characteristics of biosilica derived from marine diatom biomass of Nitzschia closterium and Thalassiosira. Chemistry, 35, 668–680.
25. Reynolds, C. S. (2006). The ecology of phytoplankton. Cambridge University Press, 435pp.
26. Sánchez-Bayo, A., López-Jiménez, P. A., García-Villén, F., & Cerdán, L. E. (2024). Review of factors influencing microalgae culture performance. Algal Research, 78, 103645.
27. Santos-Ballardo, D. U., Rossi, S., Hernández, V., Gómez, R. V., Rendón-Unceta, M. C., Caro-Corrales, J., & Valdez-Ortiz, A. (2017). A simple spectrophotometric method for biomass measurement of important microalgae species in aquaculture. Aquaculture, 448, 87–92.
28. Silveira Júnior, N., Pinto, M. F., & Costa, A. L. (2019). The role of microalgae in aquaculture: A review. Brazilian Journal of Aquatic Science and Technology, 23(2), 1–9.
29. Sipaúba‐Tavares, L. H., Scardoeli‐Truzzi, B., & Berchielli-Morais, F. A. (2017). Growth and pigment content of Chaetoceros spp. cultured under different nutrient concentrations. Brazilian Journal of Biology, 77(4), 795–803.
30. Strickland, J. D. H., & Parsons, T. R. (1972). A Practical Handbook of Seawater Analysis (2nd ed.). Fisheries Research Board of Canada, Bulletin 167.
31. Tâm, L. V., Thảo, N. P., Danh, N. C., Vi, P. T. T., & Thành, T. (2020). Application of microalgae technology for nitrogen and phosphorus removal from shrimp pond wastewater. Can Tho University Journal of Science, 58(3B), 126–131.
32. Thủy, N. T. T., Bình, M. N., & Ngọc, T. N. (2021). Effects of nutrient media and salinity on the growth of the microalga Nannochloropsis oculata. Hue University Journal of Science: Agriculture and Rural Development, 130(3A), 13–23.
33. Tuyền, N. T. T. (2021). Study on the effects of different nutrient media on the growth of the microalga Thalassiosira weissflogii. Can Tho University Journal of Science, 56(2), 45–53.
34. Tuyến, N. X. (2023). Report on feed control in shrimp broodstock and larval production. Research Institute for Aquaculture No. I.
35. VASEP. (2025). Infographic: Vietnam’s shrimp exports in the first quarter of 2024. Vietnam Association of Seafood Exporters and Producers.
36. Yi, Z., Xu, C., Benning, C., & Dellapenna, M. (2019). Artificial high-silicate medium promotes silica accumulation and pigment production in the marine diatom Phaeodactylum tricornutum. Microbial Cell Factories, 18(1), 1–12.