Effect of Ethanol (Sophia) Concentrations on Physicochemical Quality and Lipid Oxidation of Na’an Maran (Dried Timorese Beef)
Abstract:
Na’an maran is a traditional Timorese dried beef product. This experiment investigated the effects of adding Sophia (an ethanol-containing beverage) at different alcohol concentrations on the quality and potential shelf-life of Na’an maran. A completely randomized design (CRD) 4 × 5 (four treatments and five replicates each treatment). The treatments were: S0 (no added alcohol/ control): S1 (Sophia with 10% alcohol); S2 (Sophia with 12% alcohol); and S3 (Sophia with 14% alcohol). Quality characteristics assessed included pH. texture (hardness), meat microstructure, water, protein and lipid contents, TBA values, and ethanol residue. Data were analyzed using analysis of variance (ANOVA). The results showed that the addition of Sophia at 10–14% significantly reduced pH, yield, and hardness (P < 0.05). The lipid content was significantly lower in the 12–14% treatments (P < 0.05). and TBA values decreased with alcohol treatment (P < 0.05). indicates improved oxidative stability. The protein content and ethanol residue were not significantly affected. Microstructural observations revealed that alcohol-treated samples had reduced diameters of muscle fibers and collagen. Overall. The study suggests that Sophia at 10–14% alcohol concentration can improve tenderness and may help prolong the shelf life of Na’an maran by lowering pH and water-related properties and reducing lipid oxidation (TBA). Importantly. All measured ethanol residues were below the Indonesian Ulema Council (MUI) recommended limits.
KeyWords:
Na’an maran, Alcohol concentration, pH, lipid oxidation, microstructure
References:
- Arcanjo, N. M. O., Morcuende, D., Andrade, M. J., Padilla, P., Madruga, M. S., &, & Estévez, M. (2019). Bioactivities of wine components on marinated beef during aging. Journal of Functional Foods, 57(March), 19–30. https://doi.org/10.1016/j.jff.2019.03.040
- Campo, M. M., Nute, G.R., Hughes, S.I., Enser, M.., Wood, J.D.., & R. I., & Z. (2006). Flavour perception of oxidation in beef. Meat Science, 72(2), 303–311. https://doi.org/10.1016/j.meatsci.2005.07.015
- Dolgosheva, E.V., Romanova, T.N., Baimishev, R. Kh & Korosteleva, L. A. (2022). The utilization of strong alcoholic beverages while processing semi-smoked sausage The utilization of strong alcoholic beverages while processing semi-smoked sausage. IOP Conf. Series: Earth and Environmental Science, 954. https://doi.org/10.1088/1755-1315/954/1/012026
- Engez, S.T., Baskan, P., & Ergönül, B. (2012). Chemical, Textural and Sensorial Attributes of Biltong Produced through Different Manufacturing Processese. Korean J. Food Sci. Ani. Resour, 32(3), 263–267. https://doi.org/http://dx.do.org/10.5851/kosfa.2012.32.3.263
- Ferreira, V., Martins, T., Batista, E., Santos, E., Silva, F., Araújo, I., & Nascimento, M. (2013). Physicochemical and microbiological parameters of dried salted pork meat with different sodium chloride levels. 10.1590/S0101-20612013005000055 Food Science and Technology (Campinas), 33(2), 382–386. https://doi.org/10.1590/S0101-20612013005000055
- Gradinarska, D., Valkova-Yorgova, K. I.& Danov, K. R. (2022). Effect of red wine in dry fermented sausages produced with a starter culture for improving their quality and safety. Food Science and Technology, 42, e47722, 1–8. https://doi.org/https://doi.org/10.1590/fst.47722 Effect
- Han, J., Wang, Y., Wang, Y., & Hao, S., Zhang, K., Tian, J., Jin, Y. (2024). Effect of changes in the structure of myoglobin on the color of meat products. Food Materials Research, 4: e011. https://doi.org/https://doi.org/10.48130/fmr-0024-0003
- FATWA MAJELIS ULAMA INDONESIA Nomor : 40 Tahun 2018ِ, (2018).
- Istrati, D., Ciuciu, A. S., Vizireanu, C., Ionescu, A., & Carballo, J. (2015). Impact of spices and winw -based marinades on tenderness , fragmentation of myofibrillar protein and color stability in bovine bicep femoris muscle. 455–466. https://doi.org/10.1111/jtxs.12144
- Jones, M., Arnaud, E., Gouws, P., & Hoffman, L. C. (2017). Processing of South African biltong – A review. South African Journal of Animal Science, 47(6), 743–757. https://doi.org/https://doi.org/10.4314/sajas.v47i6.2
- Kaban, G. (2013). Sucuk and past ı rma : Microbiological changes and formation of volatile compounds. 95(4), 912–918. https://doi.org/10.1016/j.meatsci.2013.03.021
- Khalid, W., Maggiolino, A., Kour, J., Arshad, M. S., Aslam, N., Afzal, M. F., Meghwar, P., Zafar, K. U., De Palo, P., & Korma, S. A. (2023). Dynamic alterations in protein , sensory , chemical , and oxidative properties occurring in meat during thermal and non-thermal processing techniques : A comprehensive review. Frontiers in Nutrition, 9:1057457(January), 1–19. https://doi.org/https://10.3389/fnut.2022.105745710.3389/fnut.2022.1057457
- Kupaeva, N., Bogdanova, Y., Motovilina, A.Utyanov, D., Knyazeva, A., Knyazeva, A., Pchelkina, Vi., & Kotenkova, E. (2024). study of the effect of onion husk ethanol extract on the chemical composition and microstructure of meat pates. Theory and Practice of Meat Processing, 9(1), 75–87. https://doi.org/10.21323/2414-438X-2024-9-1-75-87
- Mediani, A., Hamezah, H. S., Jam, F. A., Mahadi, N. F., Xi, S., Chan, Y., Rohani, E. R., Hanini, N., Lah, C., Azlan, U. K., Aisyah, N., Annuar, K., Aida, N., Azman, F., Bunawan, H., Sarian, M. N., Kamal, N., & Donn, P. (2022). A comprehensive review of drying meat products and the associated effects and changes. November, 1–24. https://doi.org/https://doi.org/10.3389/fnut.2022.1057366
- Melo, A., Viegas, O., Petisca, C., Pinho, O., &, & Ferreira, I. M. P. L. V. O. (2008). Effect of Beer / Red Wine Marinades on the Formation of Heterocyclic Aromatic Amines in Pan-Fried Beef. J. Agric. Food Chem, 56(22), 10625–10632. https://doi.org/https://doi.org/10.1021/jf801837s
- Mishra, B., Mishra, J., Pati, P., & Rath, P. (2017). Dehydrated meat products: A Review. International Journal of Livestock Research ·, 7(11), 9–22. https://doi.org/10.5455/ijlr.20170812035616
- Mohd-esa, O., Hern, F.S., Ismail, A., & Yee, C. L. (2010). Antioxidant activity in different parts of roselle ( Hibiscus sabdariffa L .) extracts and potential exploitation of the seeds. Food Chemistry, 122(4), 1055–1060. https://doi.org/10.1016/j.foodchem.2010.03.074
- Momchilovа, M., Gradinarska-Ivanova, D., Yordanov, D., Zsivanovits, G. (2023). Microstructure and technological properties of cooked meat sausages prepared. Food Research, 7(4), 22–29. https://doi.org/https://doi.org/10.26656/fr.2017.7(4).1030
- Naiola, E. (2008). Mikrobia Amilolitik pada Nira dan Laru dari Pulau Timor , Nusa Tenggara Timur Amylolitic microbes of nira and laru from Timor Island , East Nusa Tenggara. Biodiversitas, 9(3), 165–168. https://doi.org/10.13057/biodiv/d090302
- National Standardization Agency of Indonesia, 2013 / Badan Standardisasi Nasional Indonesia. (2013). SNI 2908:2013, Dendeng sapi.
- Petit, T., Caro, Y., Petit, A. S., Santchurn, S. J., & Collignan, A. (2014). Physicochemical and microbiological characteristics of biltong , a traditional salted dried meat of South Africa. Meat Science, 96, 1313–1317. https://doi.org/10.1016/j.meatsci.2013.11.003
- Polak, J. E., Bartoszek, M., Lowe, A. R., Postnikov, E. B., & Chorążewski, M. A. (2020). “Antioxidant Properties of Various Alcoholic Beverages: Application of a Semiempirical Equation.,.” Analytical Chemistry, 92(2), 2145–2150. https://doi.org/. https://doi.org/10.1021/acs.analchem.9b04692
- Predrag Ikonić, Marija Jokanović, Ljiljana Petrović, Tatjana Tasić, Snežana Škaljac, Branislav Šojić, Natalija Džinić, Vladimir Tomović, Jelena Tomić, B. D. & B. I. (2016). Effect of starter culture addition and processing method on proteolysis and texture profile of traditional dry-fermented sausage Petrovská klobása. International Journal of Food Properties, 19(December), 1924–1937. https://doi.org/10.1080/10942912.2015.1089280
- Ryapushkina, J., Skovenborg, E., Astrup, A., Risbo, J., Mølskov, L., Jensen, G., & Snitkjær, P. (2016). Cooking with beer : How much alcohol is left ? International Journal of Gastronomy and Food Science, 5–6, 17–26. https://doi.org/10.1016/j.ijgfs.2016.09.001
- Shi, S., Kong, B., Wang, Y., Liu, Q., & Xia, X. (2020). Comparison of the quality of beef jerky processed by traditional and modern drying methods from different districts in Inner Mongolia. Meat Science, 163, 108080. https://doi.org/10.1016/j.meatsci.2020.108080
- Snitkjær, P., Ryapushkina, J., Skovenborg, E., Astrup, A., Mølskov, L., Jensen, M. G., & Risbo, J. (2017). Fate of ethanol during cooking of liquid foods prepared with alcoholic beverages : Theory and experimental studies. Food Chemistry. https://doi.org/10.1016/j.foodchem.2017.03.034
- Spaziani, M., Torre, M. Del, & Stecchini, M. L. (2009). Changes of physicochemical , microbiological , and textural properties during ripening of Italian low-acid sausages . Proteolysis , sensory and volatile profiles. Meat Science, 81(1), 77–85. https://doi.org/10.1016/j.meatsci.2008.06.017
- Tan, M., Ye, J., & Xie, J. (2021). Freezing-induced myofibrillar protein denaturation : Role of pH change and freezing rate. LWT, 152(5), 112381. https://doi.org/https://doi.org/10.1016/j.lwt.2021.112381
- Veselá, H., Ježek, F., Dušková, M., Kameník, J., Hulánková, R., Macharáčková, B., Brodíková, K., & Karpíšková, R. (2025). Jerky and Biltong from the Czech retail market: Microbial quality, chemical composition, and other quality characteristics. Foods, 14(21), 3792. https://doi.org/https://doi.org/10.3390/foods14213792
- Wahyuni, D., Setiyono., &, & Supadmo. (2012). Effect of anka rice addition and filler combination of wheat flour and Sweet potato flour on beef sausage quality. Buletin Peternakan, 36(3), 181–192. https://doi.org/https://doi.org/10.21059/buletinpeternak.v36i3.1627
- Yulianti, C. H. (2014). Testing the difference in alcohol content in rice tape, black glutinous rice, and cassava (Uji beda kadar alkohol pada tape beras). Jurnal Teknika, 6(1), 531–536. https://www.scribd.com/doc/310447286/Analisis-Tape
- Zioud, A., Hajji, W., Lobón, S., Joy, M., Bertolin, J. R., Smeti, S., Chabbouh, M., Bellagha, S., & Essid, I. (2023). Effects of Drying Methods on Chemical Composition , Lipid Oxidation , and Fatty Acid Profile of a Traditional Dried Meat Kaddid. Foods, 12(20), 3837. https://doi.org/https://doi.org/10.3390/foods12203837