Nanomaterial-Based Biosensors for Early Breast Cancer Detection: A Comprehensive Review of Technologies and Biomarkers
Abstract:
Nanomaterial based biosensor have emerged apromising approach that demonstrates great progress in the diagnosis of breast cancer with increased sensitivity and selectivity compared to conventional detection methods. These nanomaterials may allow for combinatorial detection capabilities that could provide simultaneous identification of multiple biomarkers in order to reach an accurate tumor classification, by using quantum dot imaging and carbon nanotube electrical sensing. However, this field faces challenges that must be addressed before widespread application of clinical. Key among these is the need to standardize methods and protocols to ensure reproducibility and minimize non-specific correlations. Furthermore, ensuring the nanomaterial,s safety and bio-compatibility, as well as the toxicity of a diagnostic methods, are essential elements for their success in future patient care.
KeyWords:
Nanomaterial, biosensors, breast cancer, detection, biomarkers, Optical Biosensors, Electrochemical Biosensors
References:
- Xiaoqing Li , Fanghui Ma , Minghui Yang , Jianglin Zhang, Nanomaterial based analytical methods for breast cancer biomarker detection, Materials Today Advances, Volume 14, June 2022, 100219.
- Alagheband Y, Jafari gharabaghlou D, Imani M, Mousazadeh H, Dadashpour M, Firouzi-Amandi A, Zarghami N. Design and fabrication of a dual-drug loaded nano-platform for synergistic anticancer and cytotoxicity effects on the expression of leptin in lung cancer treatment. J Drug Deliv Sci Technol. 2022;73:103389.
- Firouzi Amandi A, Jokar E, Eslami M, Dadashpour M, Rezaie M, Yazdani Y, Nejati B. Enhanced anti-cancer effect of artemisinin-and curcumin-loaded niosomal nanoparticles against human colon cancer cells. Med Oncol. 2023;40(6):170.
- Firouzi-Amandi A, Tarahomi M, Rahmani-Youshanlouei H, Heris RM, Jafari-Gharabaghlou D, Zarghami N, Dadashpour M. Development, characterization, and in vitro evaluation of cytotoxic activity of Rutin loaded PCL-PEG nanoparticles against Skov3 ovarian cancer cell. Asian Pac J Cancer Prevention: APJCP. 2022;23(6):1951.
- Yang F, He Q, Dai X, Zhang X, Song D. The potential role of nanomedicine in the treatment of breast cancer to overcome the obstacles of current therapies. Front Pharmacol 2023, 14.
- Tagde P, Najda A, Nagpal K, Kulkarni GT, Shah M, Ullah O, Balant S, Rahman MH. Nanomedicine-based delivery strategies for breast Cancer Treatment and Management. Int J Mol Sci 2022, 23(5).
- Bhakta S., Mishra P. Molecularly Imprinted Polymer-Based Sensors for Cancer Biomarker Detection. Sens. Actuators Rep. 2021;3:100061.
- MagarH. S.HassanR. Y. A. Mulchandani electrochemical impedance spectroscopy (EIS): principles, construction, and biosensing applications. Sensors21, 2021,6578. 10.3390/s21196578.
- Gajdosova, V.; Lorencova, L.; Prochazka, M.; Omastova, M.; Micusik, M.; Prochazkova, S.; Kveton, F.; Jerigova, M.; Velic, D.; Kasak, P.; et al. Remarkable differences in the voltammetric response towards hydrogen peroxide, oxygen and Ru(NH(3))(6)(3+) of electrode interfaces modified with HF or LiF-HCl etched Ti(3)C(2)T(x) MXene. Mikrochim. Acta 2019, 187, 52.
- Safari M, Moghaddam A, Salehi Moghaddam A, et al. Carbon-based biosensors from graphene family to carbon dots: Aviewpoint in cancer detection. Talanta. 2023; 258: 124399.
- Lorena Gárate-Vélez & Mildred Quintana, Carbon-based nanomaterials: interactions with cells, brain therapies, and neural sensing., Journal of Materials Science: Materials in Engineering, Volume 20, 2025,article number 23.
- Li CH, Chan MH, Chang YC, et al. Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination. Molecules. 2023; 28(1): 364.
- Atapour A, Khajehzadeh H, Shafie M, et al. Gold nanoparticle-based aptasensors: A promising perspective for early-stage detection of cancer biomarkers. Materials Today Communications. 2022; 30: 103181.
- Choi N, Dang H, Das A, et al. SERS biosensors for ultrasensitive detection of multiple biomarkers expressed in cancer cells.Biosensors and Bioelectronics. 2020; 164: 112326.
- Song C, Guo S, Jin S, et al. Biomarkers Determination Based on Surface-Enhanced Raman Scattering. Chemosensors. 2020;8(4): 118.
- M.S. Dresselhaus, Y.-M. Lin, T. Koga, S.B. Cronin, O. Rabin, M.R. Black, G. Dresselhaus Low dimensional thermoelectricity
- T.M. Tritt (Ed.), Semiconductors and Semimetals: Recent Trends in Thermoelectric Materials Research III, vol. 71, Academic Press, San Diego, CA (2001), pp. 1-121.
- R. Saito, G. Dresselhaus, M.S. Dresselhaus,Physical Properties of Carbon Nanotubes,Imperial College Press, London (1998).
- Smith R, Geary SM, Salem AK. Silicon Nanowires and Their Impact on Cancer Detection and Monitoring. ACS Applied Nano Materials. 2020; 3(9): 8522-–8536.
- Farzin A, Etesami SA, Quint J, et al. Magnetic Nanoparticles in Cancer Therapy and Diagnosis. Advanced Healthcare Materials. 2020; 9(9).
- James Chun Lam Chow, 8 - Synthesis and applications of functionalized nanoparticles in biomedicine and radiotherapy, Additive Manufacturing with Functionalized Nanomaterials,Micro and Nano Technologies,2021, Pages 193-218.
- Ghosh G. Early detection of cancer: Focus on antibody coated metal and magnetic nanoparticle-based biosensors. Sensors International. 2020; 1: 100050.
- Qureshi A, Tufani A, Corapcioglu G, et al. CdSe/CdS/ZnS nanocrystals decorated with Fe3O4 nanoparticles for point-of-care optomagnetic detection of cancer biomarker in serum. Sensors and Actuators B: Chemical. 2020; 321: 128431.
- Ziegler JM, Andoni I, Choi EJ, et al. Sensors Based Upon Nanowires, Nanotubes, and Nanoribbons: 2016–2020. AnalyticalChemistry. 2020; 93(1): 124–166.
- R.A. Karim, Y. Reda, A.A. Fattah, Review-Nanostructured materials-based nanosensors, J. Electrochem. Soc. 167 (2020), 037554.
- H. Barabadi, M. Najafi, H. Samadian, A. Azarnezhad, H. Vahidi, M.A. Mahjoub, M. Koohiyan, A. Ahmadi, A systematic review of the genotoxicity and antigenotoxicity of biologically synthesized metallic nanomaterials: are green nanoparticles safe, Enough for Clinical Marketing. Medicina. 55 (8) (2019) 439.
- Sumit Malik , Joginder Singh a, Rohit Goyat a , Yajvinder Saharan a , Vivek Chaudhry a , Ahmad Umar ,Ahmed A. Ibrahim , Sheikh Akbar , Sadia Ameen , Sotirios Baskout, Nanomaterials-based biosensor and their applications: A review, Heliyon, 2023,Volume 9, Issue 9e19929 .
- Tijjani Adam , Subash C.B. Gopinath , Nanosensors: Recent perspectives on attainments and future promise of downstream applications, Process Biochemistry,Volume 117, 2022, Pages 153-173.
- R. K. Saini, L. P. Bagri, and A. K. Bajpai, in New Pesticides and Soil Sensors, ed. A. Grumezescu (Elsevier, Amsterdam) Chap. 14 ,2017.
- Ruixue Zhu , Tatiana Avsievich , Alexey Popov , Alexander Bykov , Igor Meglinski, In vivo nano-biosensing element of red blood cell-mediated delivery, Biosensors and Bioelectronics,Volume 175, 2021, 112845.
- Scognamiglio V., Arduini F., Palleschi G., Rea G. Biosensing technology for sustainable food safety. Trends Anal Chem. 2014;62:1–10.
- Parikha Mehrotra, Biosensors and their applications – A review, J Oral Biol Craniofac Res. 2016;6(2):153–159.
- Leatherbarrow R.J., Edwards P.R. Analysis of molecular recognition using optical biosensors. Curr Opin Chem Biol. 1999;3:544–547.
- Guda, M. A., Alkaabiº, Z. S., Albayati, F. S., Alduhaidahawi, F. J., & Almayahi, B. A. Anatomical And Sclerophilic Traits Variation ln Two Varieties Of Olive Plants (Olea europaea L) Growing Under Climate Changes ln Various.season of the year. Indian Journal of Environmental protection,Vol.40,No.1Junuary 20,77-83.
- Guda, M.A., H.mutlag, N., Naser, A.S., Algburi, S., Green Synthesis Of Silver Nanoparticles Using Aqueous Extract Of Schanginia Aegyptiaca Wild Plant, And Study Antifungal Activity Against Some Pathogenic Fungi, Library Progress International, 2024, 44(2s), pp. 1773–1783
- Chen H, et al. Anti-biofilm nanomaterials. Microb Pathog. 2024;188:106789.
- Naresh, V. ∙ Lee, N.,A review on biosensors and recent development of nanostructured materials-enabled biosensors,Sensors. 2021; 21(4):1109.
- Karunakaran, C. ∙ Rajkumar, R. ∙ Bhargava, K.,Introduction to biosensors,Biosensors and Bioelectronics,Elsevier, 2015; 1-68.
- Wang B, Akiba U, Anzai J ichi. Recent Progress in Nanomaterial-Based Electrochemical Biosensors for Cancer Biomarkers: A Review. Molecules. 2017; 22(7): 1048.
- Kerman K, Saito M, Tamiya E, et al. Nanomaterial-based electrochemical biosensors for medical applications. TrAC Trends in Analytical Chemistry. 2008; 27(7): 585–592.
- Mokhtarzadeh A, Eivazzadeh-Keihan R, Pashazadeh P, et al. Nanomaterial-based biosensors for detection of pathogenic virus. TrAC Trends in Analytical Chemistry. 2017; 97: 445–457.
- Farzin L, Shamsipur M, Samandari L, et al. Advances in the design of nanomaterial-based electrochemical affinity andenzymatic biosensors for metabolic biomarkers: A review. Microchimica Acta. 2018; 185(5): 276.
- Guda, M.A., Response of antioxidant in some plants to iron oxide nanoparticles, AIP Conference Proceedings, 2023, 2977(1), 040144 https://doi.org/10.1063/5.0182305
- Husayn, D.M., Guda, M.A., Response of some wild plants in antioxidant enzymes by zinc oxide nanoparticles, AIP Conference Proceedings, 2023, 2787(1), 090049
- Husayn, D.M., Guda, M.A. , Effect of zinc oxide nanoparticles on biomarkers of chlorophyll and carotene in some wild plants , AIP Conference Proceedings, 2023, 2787(1), 090035
- Owied, O.A.-R., Guda, M.A.M., Taher, H.I., Abdulhussein, M.A.A., Plants anatomically engineered by nanomaterials, Bionatura, 2023, 8(2), 44
- Alabassi, M.M., Guda, M.A., Muhammed, M.A., The removal efficiency of natural nano-coagulant produced from Phragmites communis, Schanginia aegyptiaca and Portulaca oleracea in wastewater treatment , International Journal of Aquatic Biology, 2022, 10(2), pp. 181–186
- Ali, M., Othman, M., Guda, M., Abojassim, A., Almayahi, B., Effect of Climate Characteristics in the Scientific Levels of Students: An Applied Study on the City of Najaf, Journal of Physics: Conference Series, 2020, 1530(1), 012142
- Al Bayati, F.S., Al Naqeeb, N.A., Guda, M.A., Almansoori, D.H.H., Use of phytoplankton enzyme in the artificial lake as indicator of pollution , Journal of Chemical Health Risks, 2020, 10(4), pp. 353–359
- Guda, M.A., Yoness, A.Sh., Thajeel, Z.H., Almayahi, B.A., Comparison between two halophyte species for potential removal of salt under different soil salinity conditions, Indian Journal of Environmental Protection, 2019, 39(12), pp. 1084–1090
- Mutlag, N.H., Juda, M.A., Hussein, M.L., Hassan, H.N., Assessment of Bombax ceiba Leaves Extract and Pleurotus ostreatus Fungus Filtrate on treatment of some Isolated Dermatophytic Fungi, Journal of Physics: Conference Series, 2019, 1294(7), 072022
- Guda, M.A., Younus, A.S., Altamimi, A.J.T., ... Al-Edhari, A.H.M., Alduhaidahawi, F.J., Effect of Irrigation with Magnetized Water on Some Vegetative and Flowering Parameters of Wheat (Triticum aestivum L.) Cultivar IP A 99, ARPN Journal of Engineering and Applied Sciences, 2019, 14(2), pp. 5466–5470
- Guda, M. A., & Mutlag, N. H. (2024). Green Synthesis Of Silver Nanoparticles Using Aqueous Extract Of Schanginia Aegyptiaca Wild Plant, And Study Antifungal Activity Against Some Pathogenic Fungi. Library Progress International, 44(2s), 1773-1783.
- Hamad, M. O., Rahi, A. Y., & Guda, M. A. (2023). Study of Staphylococcus spp. isolated from Clinical Cases and determination of its multidrugresistant to antibiotics and phytosynthesized ZnO (NPs). Cardiometry, (26), 227-235.
- Hammad, M. O., & Guda, M. A. (2023). Effect of Phytosynthesis silver oxide nanoparticles on multidrug-resistant Klebsiella pneumonia isolated from children. Eur. Chem. Bull, 12(1), 1655-1669.
- Eqbal Yousif Abed1, Hanan Fadhil Kadhim2, Muthik A. Guda .(2025)The Effects of Climate Change on Public Health, Contemporary Research Analysis Journal,8(2),p 500-503.
- DOI: 10.55677/CRAJ/05-2025-Vol02I08
- Saba Abd Al-Mutleb Hamood1, Douaa Abdulrazzaq Khaleel2, Muthik A Guda,(2025), Harnessing Artificial Intelligence to Revolutionize Public Health: Innovations in Prevention, Monitoring, and Policy Decision-Making, Contemporary Research Analysis Journal,8(2),p: 504-509.
- DOI: 10.55677/CRAJ/06-2025-Vol02I08
- Hanan Fadhil Kadhim1, Eqbal Yousif Abed2, Muthik A. Guda3.(2025). Climate Change and Its Influence on Anatomical and Physiological Features of Pomegranate (Punica Granatum L.) Leaves, Contemporary Research Analysis Journal 8(2)p: 510-515
- Alhasnawi Radhi Jubair Nasser, Guda Muslim Abd Muthik, Al-Bayati Sami Ammar (2025). Effect of Nano-silver Fertilization on the Tolerance of Salt Stress by Irrigation with Saline Water in Faba Bean (Vicia faba L.) . Indian Journal of Agricultural Research. (): . doi: 10.18805/IJARe.AF-968.
- Ahmed, A. Q., & Guda, M. A. (2025). Comparison and characterization of green synthesized silver oxide nanoparticles using Ziziphus spina-christi plant extract and AgO NPs by femtosecond laser ablation technique. Contemporary Research Analysis Journal, 2(12), 762–769. https://doi.org/10.55677/CRAJ/02-2025-Vol02I12
- Alshebly, E. A. J., & Guda, M. A. (2026, March). Green synthesis of silver oxide nanoparticles and their effect on inhibition growth of bacteria. In AIP Conference Proceedings (Vol. 3396, No. 1, p. 030008). AIP Publishing LLC. https://doi.org/10.1063/5.0318556
- Alsultan, H. K. A. A. K., & Guda, M. A. (2026, March). Green synthesis and characterization of zirconium oxide nanoparticles using Matricaria Chamomilla L. plant extracts for antibacterial application. In AIP Conference Proceedings (Vol. 3396, No. 1, p. 080001). AIP Publishing LLC. https://doi.org/10.1063/5.0318554
- Nasser Jubair Radhi Alhasnawi1, Muthik Abd Muslim Guda, Ammar Sami Al-Bayat. (2025). Effect of Nano-silver Fertilization on the Tolerance of Salt Stress by Irrigation with Saline Water in Faba Bean (Vicia faba L.). Indian Journal of Agricultural Research. https://doi.org/ 10.18805/IJARe.AF-968