Trichoderma and Bacillus on Faba-Bean Growth and Yield Under Soil-Borne Disease Pressure
Abstract:
Faba‑bean (Vicia faba L.) production serves human consumption and is critical for food security; however, yields can become exceptionally low due to soil‑borne diseases, poor fertility and adverse climatic factors. This study evaluated the effect of seed inoculation with the fungus Trichoderma spp., the bacterium Bacillus subtilis, and their dual‑consortium on disease suppression and yield improvement in faba‑bean under Bolivian Andean Altiplano conditions. A randomized complete‑block design with four treatments (Trichoderma alone, B. subtilis alone, consortium of both, uninoculated control) was employed. At the start of the trial, rice‑trap monitoring identified three major soil pathogens (Phytophthora spp., Fusarium spp., Rhizoctonia spp.). At crop maturity, only the consortium treatment resulted in the consistent detection of Trichoderma + B. subtilis, along with suppression of Phytophthora. The consortium treatment maximised pods per plant (21 pods), seeds per pod (3), root weight (≈ 68 g) and stems per plant (8), achieving a total pod yield of 29.25 t ha-1. These results demonstrate that the Trichoderma-B. subtilis consortium is a viable and sustainable technological alternative for increasing faba‑bean productivity under the challenging soil and climatic conditions of the Bolivian highlands. Future work should quantify microbial population dynamics, elucidate mechanisms of action and evaluate performance across varieties and management systems.
KeyWords:
biological control, microbial consortium, seed inoculation, sustainable agriculture, Vicia faba.
References:
- ABDELAZIZ, A. M.; HASHEM, A. H.; EL-SAYYAD, G. S.; EL-WAKIL, D. A.; SELIM, S.; ALKHALIFAH, D. H. M.; ATTIA, M. S. Biocontrol of soil borne diseases by plant growth promoting rhizobacteria. Tropical Plant Pathology, v. 48, n. 2, p. 105-127, 2023. https://doi.org/10.1007/s40858-022-00544-7
- ABDULLAH, N. S.; DONI, F.; MISPAN, M. S.; SAIMAN, M. Z.; YUSUF, Y. M.; OKE, M. A.; SUHAIMI, N. S. M. Harnessing Trichoderma in agriculture for productivity and sustainability. Agronomy, v. 11, n. 12, p. 2559, 2021. https://doi.org/10.3390/agronomy11122559
- ALI, Q.; ALI, M.; JING, H.; HUSSAIN, A.; MANGHWAR, H.; ALI, M.; RAZA, W.; MUNDRA, S. Power of plant microbiome: a sustainable approach for agricultural resilience. Plant Stress, v. 14, p. 100681, 2024.https://doi.org/10.1016/j.stress.2024.100681
- ALOO, B. N.; TRIPATHI, V.; MAKUMBA, B. A.; MBEGA, E. R. Plant growth-promoting rhizobacterial biofertilizers for crop production: the past, present, and future. Frontiers in Plant Science, v. 13, p. 1002448, 2022.https://doi.org/10.3389/fpls.2022.1002448
- ALORI, E. T.; GLICK, B. R.; BABALOLA, O. O. Microbial phosphorus solubilization and its potential for use in sustainable agriculture. Frontiers in Microbiology, v. 8, p. 971, 2017. https://doi.org/10.3389/fmicb.2017.00971
- ALTIERI, M.; NICHOLLS, C. Biodiversity and pest management in agroecosystems. 2. ed. CRC Press, 2004.https://doi.org/10.1201/9781482277937
- ATTIA, M. S., El-WAKIL, D. A., HASHEM, A. H., Al-ASKAR, A. A., AbdELGAWAD, H., ALOTAIBI, R. S., ABDEL-KADER, S. A., & ABDELAZIZ, A. M. (2025). Investigating the activity of Bacillus subtilis and Trichoderma harzianum to mitigate Fusarium wilt disease of diverse cultivars of Vicia faba. Scientific Reports, 15(1), 16093.https://doi.org/10.1038/s41598-025-99381-2
- BROTMAN, Y.; LANDAU, U.; CUADROS-INOSTROZA, Á.; TAKAYUKI, T.; FERNIE, A. R.; CHET, I.; VITERBO, A.; WILLMITZER, L. Trichoderma-plant root colonization: escaping early plant defense responses and activation of the antioxidant machinery for saline stress tolerance. PLoS Pathogens, v. 9, n. 3, e1003221, 2013.https://doi.org/10.1371/journal.ppat.1003221
- BUCKLEY, N. E.; AVILA-SAKAR, G. Reproduction, growth, and defense trade-offs vary with gender and reproductive allocation in Ilex glabra (Aquifoliaceae). American Journal of Botany, v. 100, n. 2, p. 357-364, 2013.https://doi.org/10.3732/ajb.1200603
- CARRIÓN, V. J.; PEREZ-JARAMILLO, J.; CORDOVEZ, V.; TRACANNA, V.; DE HOLLANDER, M.; RUIZ-BUCK, D.; MENDES, L. W.; VAN IJCKEN, W. F. J.; GOMEZ-EXPOSITO, R.; ELSAYED, S. S.; MOHANRAJU, P.; ARIFAH, A.; VAN DER OOST, J.; PAULSON, J. N.; MENDES, R.; VAN WEZEL, G. P.; MEDEMA, M. H.; RAAIJMAKERS, J. M. Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome. Science, v. 366, n. 6465, p. 606-612, 2019. https://doi.org/10.1126/science.aaw9285
- CASTILLO, E.; SILES, M.; RÍOS, R.; GABRIEL, J. Inheritance of the number of pods per node and its relationship with related characteristics in pea (Pisum sativum L.). Journal of the Selva Andina Biosphere, v. 2, n. 1, p. 2-14, 2014. https://doi.org/10.36610/j.jsab.2014.020100002
- CHEN, Y.; FU, Y.; XIA, Y.; MIAO, Y.; SHAO, J.; XUAN, W.; LIU, Y.; XUN, W.; YAN, Q.; SHEN, Q.; ZHANG, R. Trichoderma-secreted anthranilic acid promotes lateral root development via auxin signaling and RBOHF-induced endodermal cell wall remodeling. Cell Reports, v. 43, n. 4, p. 114030, 2024. https://doi.org/10.1016/j.celrep.2024.114030
- CONTRERAS-CORNEJO, H. A.; MACÍAS-RODRÍGUEZ, L.; DEL-VAL, E.; LARSEN, J. Ecological functions of Trichoderma spp. and their secondary metabolites in the rhizosphere: interactions with plants. FEMS Microbiology Ecology, v. 92, n. 4, fiw036, 2016. https://doi.org/10.1093/femsec/fiw036
- CUERVO, Y.; ESPADA, M.; ZITA, G. Manual de prácticas de fitopatología. Universidad Nacional Autónoma de México, 2019.
- DA SILVA, D. M. M.; SANTOS, C. C.; WAGNER, F. E.; MARTINS, L. O. M.; OZÓRIO, J. P. A.; DA SILVA, O. A.; RIBEIRO, D. M.; SCALON, S. P. Q. Seed biopriming with Parachlorella, Bacillus subtilis, and Trichoderma harzianum alleviates the effects of salinity in soybean. BMC Plant Biology, v. 24, p. 1149, 2024. https://doi.org/10.1186/s12870-024-05646-9
- DE CORATO, U. Disease-suppressive compost enhances natural soil suppressiveness against soil-borne plant pathogens: a critical review. Rhizosphere, v. 13, p. 100192, 2020. https://doi.org/10.1016/j.rhisph.2020.100192
- EGAMBERDIEVA, D.; WIRTH, S.; ALQARAWI, A. A.; ABD-ALLAH, E. F.; HASHEM, A. Phytohormones and beneficial microbes: essential components for plants to balance growth and stress responses. Frontiers in Microbiology, v. 8, p. 2104, 2017. https://doi.org/10.3389/fmicb.2017.02104
- FAOSTAT. FAOSTAT. 2025. Disponível em: https://www.fao.org/faostat/es/#data/QCL
- GALBIERI, R.; ALVES DE OLIVEIRA, J.; FRANÇA NEGRI, B.; SOUZA BOLDT, A.; DOS SANTOS RIZZI, U.; BELOT, J. L. Bacillus subtilis as growth-promoting rhizobacteria co-inoculated on Bradyrhizobium-treated soybean seeds in the planting furrow. Revista Ceres, v. 70, n. 6, 2023. https://doi.org/10.1590/0034-737X202370060001
- GARCÉS-FIALLOS, F. R.; VERA-ALCÍVAR, Á. M. Enfermedades y componentes de rendimiento en líneas de fréjol bajo tres densidades de siembra [Diseases and yield components in bean lines under three planting densities]. Agronomía Mesoamericana, v. 25, n. 1, p. 169, 2014. https://doi.org/10.15517/am.v25i1.14492
- HARMAN, G. E. Overview of mechanisms and uses of Trichoderma spp. Phytopathology, v. 96, n. 2, p. 190-194, 2006. https://doi.org/10.1094/PHYTO-96-0190
- HASHEM, A.; TABASSUM, B.; FATHI ABD-ALLAH, E. Bacillus subtilis: a plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi Journal of Biological Sciences, v. 26, n. 6, p. 1291-1297, 2019.https://doi.org/10.1016/j.sjbs.2019.05.004
- ILLESCAS, M.; PEDRERO-MÉNDEZ, A.; PITORINI-BOVOLINI, M.; HERMOSA, R.; MONTE, E. Phytohormone production profiles in Trichoderma species and their relationship to wheat plant responses to water stress. Pathogens, v. 10, n. 8, p. 991, 2021. https://doi.org/10.3390/pathogens10080991
- INE. Agricultura. 2025. Disponível em:https://www.ine.gob.bo/index.php/estadisticas-economicas/agropecuaria/agricultura-introduccion/
- IQBAL, Z.; AHMAD, M.; RAZA, M. A.; HILGER, T.; RASCHE, F. Phosphate-solubilizing Bacillus sp. modulate soil exoenzyme activities and improve wheat growth. Microbial Ecology, v. 87, n. 1, p. 31, 2024. https://doi.org/10.1007/s00248-023-02340-5
- JENSEN, C. N. G.; PANG, J. K. Y.; GOTTARDI, M.; KRAČUN, S. K.; SVENDSEN, B. A.; NIELSEN, K. F.; KOVÁCS, Á. T.; MOELBAK, L.; FIMOGNARI, L.; HUSTED, S.; SCHULZ, A. Bacillus subtilis promotes plant phosphorus (P) acquisition through P solubilization and stimulation of root and root hair growth. Physiologia Plantarum, v. 176, n. 3, e14338, 2024. https://doi.org/10.1111/ppl.14338
- KOUR, D.; RANA, K. L.; YADAV, A. N.; YADAV, N.; KUMAR, M.; KUMAR, V.; VYAS, P.; DHALIWAL, H. S.; SAXENA, A. K. Microbial biofertilizers: bioresources and eco-friendly technologies for agricultural and environmental sustainability. Biocatalysis and Agricultural Biotechnology, v. 23, p. 101487, 2020. https://doi.org/10.1016/j.bcab.2019.101487
- KREDICS, L.; BÜCHNER, R.; BALÁZS, D.; ALLAGA, H.; KEDVES, O.; RACIĆ, G.; VÁGVÖLGYI, C.; SIPOS, G. Recent advances in the use of Trichoderma-containing multicomponent microbial inoculants for pathogen control and plant growth promotion. World Journal of Microbiology and Biotechnology, v. 40, p. 162, 2024. https://doi.org/10.1007/s11274-024-03965-5
- LIU, Y.; PATKO, D.; ENGELHARDT, I.; GEORGE, T. S.; STANLEY-WALL, N. R.; LADMIRAL, V.; AMEDURI, B.; DANIELL, T. J.; HOLDEN, N.; MACDONALD, M. P.; DUPUY, L. X. Plant-environment microscopy tracks interactions of Bacillus subtilis with plant roots across the entire rhizosphere. Proceedings of the National Academy of Sciences, v. 118, n. 48, e2109176118, 2021. https://doi.org/10.1073/pnas.2109176118
- LÓPEZ-BUCIO, J.; PELAGIO-FLORES, R.; HERRERA-ESTRELLA, A. Trichoderma as biostimulant: exploiting the multilevel properties of a plant beneficial fungus. Scientia Horticulturae, v. 196, p. 109-123, 2015. https://doi.org/10.1016/j.scienta.2015.08.043
- MAALOUF, F.; HU, J.; O’SULLIVAN, D. M.; ZONG, X.; HAMWIEH, A.; KUMAR, S.; BAUM, M. Breeding and genomics status in faba bean (Vicia faba). Plant Breeding, v. 138, n. 4, p. 465-473, 2019. https://doi.org/10.1111/pbr.12644
- MAITRA, S.; BRESTIC, M.; BHADRA, P.; SHANKAR, T.; PRAHARAJ, S.; PALAI, J. B.; SHAH, M. M. R.; BAREK, V.; ONDRISIK, P.; SKALICKÝ, M.; HOSSAIN, A. Bioinoculants—natural biological resources for sustainable plant production. Microorganisms, v. 10, n. 1, p. 51, 2021. https://doi.org/10.3390/microorganisms10010051
- MAPCARTA. Tacamara. s.d. Disponível em: https://mapcarta.com/20153224
- MARTÍNEZ-MEDINA, A.; FERNÁNDEZ, I.; SÁNCHEZ-GUZMÁN, M. J.; JUNG, S. C.; PASCUAL, J. A.; POZO, M. J. Deciphering the hormonal signalling network behind the systemic resistance induced by Trichoderma harzianum in tomato. Frontiers in Plant Science, v. 4, 2013. https://doi.org/10.3389/fpls.2013.00206
- MENDOZA-MENDOZA, A.; ZAID, R.; LAWRY, R.; HERMOSA, R.; MONTE, E.; HORWITZ, B. A.; MUKHERJEE, P. K. Molecular dialogues between Trichoderma and roots: role of the fungal secretome. Fungal Biology Reviews, v. 32, n. 2, p. 62-85, 2018. https://doi.org/10.1016/j.fbr.2017.12.001
- OAP. Observatorio Agroambiental y Productivo. 2025. Disponível em: https://observatorioagro.gob.bo/
- PÉREZ-MONTAÑO, F.; ALÍAS-VILLEGAS, C.; BELLOGÍN, R. A.; DEL CERRO, P.; ESPUNY, M. R.; JIMÉNEZ-GUERRERO, I.; LÓPEZ-BAENA, F. J.; OLLERO, F. J.; CUBO, T. Plant growth promotion in cereal and leguminous agricultural important plants: from microorganism capacities to crop production. Microbiological Research, v. 169, n. 5-6, p. 325-336, 2014. https://doi.org/10.1016/j.micres.2013.09.011
- POVEDA, J. Trichoderma as biocontrol agent against pests: new uses for a mycoparasite. Biological Control, v. 159, p. 104634, 2021. https://doi.org/10.1016/j.biocontrol.2021.104634
- RANA, K. L.; KOUR, D.; KAUR, T.; SHEIKH, I.; YADAV, A. N.; KUMAR, V.; SUMAN, A.; DHALIWAL, H. S. Endophytic microbes from diverse wheat genotypes and their potential biotechnological applications in plant growth promotion and nutrient uptake. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, v. 90, n. 5, p. 969-979, 2020. https://doi.org/10.1007/s40011-020-01168-0
- SALWAN, R., SHARMA, A., KAUR, R., SHARMA, R., & SHARMA, V. (2022). The riddles of Trichoderma-induced plant immunity. Biological Control, 174, 105037. https://doi.org/10.1016/j.biocontrol.2022.105037
- SAHA, M.; SARKAR, S.; SARKAR, B.; SHARMA, B. K.; BHATTACHARJEE, S.; TRIBEDI, P. Microbial siderophores and their potential applications: a review. Environmental Science and Pollution Research, v. 23, n. 5, p. 3984-3999, 2016. https://doi.org/10.1007/s11356-015-4294-0
- SENAMHI. 2025. Disponível em: https://senamhi.gob.bo/index.php
- SENKOVS, M., NIKOLAJEVA, V., MAKARENKOVA, G., MAKAROVA, S., & GRUBE, M. (2021). Influence of Trichoderma asperellum and Bacillus subtilis as biocontrol and plant growth promoting agents on soil microbiota. Annals of Microbiology, 71, 34. https://doi.org/10.1186/s13213-021-01647-3
- SHAFI, Z., SHAHID, M., SINGH, A., RAJ, G. B., & RASOOL, A. (2026). Engineering Trichoderma-mediated plant defense against bacterial phytopathogens: Micro- and nanobiotechnological strategies. AIMS Microbiology, 12(1), 27–62. https://doi.org/10.3934/microbiol.2026002
- SHI, J.; WANG, X.; WANG, E. Mycorrhizal symbiosis in plant growth and stress adaptation: from genes to ecosystems. Annual Review of Plant Biology, v. 74, n. 1, p. 569-607, 2023. https://doi.org/10.1146/annurev-arplant-061722-090342
- SILVA, D. M. M., SANTOS, C. C., WAGNER, F. E., OLIVEIRA, A. L. M., SOUZA, R. S., & SANTOS, A. C. A. Seed biopriming with Parachlorella, Bacillus subtilis, and Trichoderma harzianum alleviates the effects of salinity in soybean. BMC Plant Biology, 24, 1149, 2024. https://doi.org/10.1186/s12870-024-05646-9
- SILVA, G. A.; PICANÇO, M. C.; BACCI, L.; CRESPO, A. L. B.; ROSADO, J. F.; GUEDES, R. N. C. Control failure likelihood and spatial dependence of insecticide resistance in the tomato pinworm, Tuta absoluta. Pest Management Science, v. 67, n. 8, p. 913-920, 2011. https://doi.org/10.1002/ps.2131
- SINGH, G.; PUJARI, M. Bacillus subtilis as a plant-growth-promoting rhizobacteria: a review. Plant Archives, v. 22, n. 2, p. 100-109, 2022. https://doi.org/10.51470/PLANTARCHIVES.2022.v22.no2.018
- TAHIR, H. A. S.; GU, Q.; WU, H.; RAZA, W.; HANIF, A.; WU, L.; COLMAN, M. V.; GAO, X. Plant growth promotion by volatile organic compounds produced by Bacillus subtilis SYST2. Frontiers in Microbiology, v. 8, p. 171, 2017. https://doi.org/10.3389/fmicb.2017.00171
- TORREZ, V. Seguridad alimentaria en el ayllu Corpa Altiplano norte de Bolivia: situación, análisis y lineamientos para su gestión territorial. Proyecto IDH-UMSA 2015-2018, 2019. ISBN 978-99954-1-921-9
- VIRACOCHA-MAMANI, P. M.; CADENA-MIRANDA, F. A. Incorporación de Trichoderma harzianum para la reducción del ataque de la tristeza del pimiento (Phytophthora capsici). Revista de Investigación e Innovación Agropecuaria y de Recursos Naturales, v. 10, n. 3, p. 56-63, 2023. https://doi.org/10.53287/iymb3882sv61c
- WANG, Z.; LI, Y.; ZHUANG, L.; YU, Y.; LIU, J.; ZHANG, L.; GAO, Z.; WU, Y.; GAO, W.; DING, G.-C.; WANG, Q. A rhizosphere-derived consortium of Bacillus subtilis and Trichoderma harzianum suppresses common scab of potato and increases yield. Computational and Structural Biotechnology Journal, v. 17, p. 645-653, 2019.https://doi.org/10.1016/j.csbj.2019.05.003
- ZHAO, L.; WANG, Y.; KONG, S. Effects of Trichoderma asperellum and its siderophores on endogenous auxin in Arabidopsis thaliana under iron-deficiency stress. International Microbiology, v. 23, n. 4, p. 501-509, 2020.https://doi.org/10.1007/s10123-020-00122-4
- ZHU, L.; HUANG, J.; LU, X.; ZHOU, C. Development of plant systemic resistance by beneficial rhizobacteria: recognition, initiation, elicitation and regulation. Frontiers in Plant Science, v. 13, p. 952397, 2022. https://doi.org/10.3389/fpls.2022.952397