Entomo-Pathogenic Nematode: An Effective and Eco-Friendly Alternative to Synthetic Pesticides. A Review

Author's Information:

Rajab, Y. S.

Department of Crop Production Technology, Federal Polytechnic, Bali. PMB 05

Aji, M. B.

Department of Crop Protection, Modibbo Adama University, Yola

Vol 05 No 07 (2026):Volume 05 Issue 07 July 2026

Page No.: 577-581

Abstract:

Entomopathogenic nematodes (EPNs) belong to the category of nematodes living in the soil, which also parasitize and kill insects. They operate in conjunction with specific bacteria and are commonly used in biocontrol because they are effective against a wide range of insect pests, host specific, environmentally safe and compatible with integrated pest management (IPM) strategies. They are obligate parasites of insects that belong to the families Steinernematidae and Heterorhabditidae. EPNs are commonly used in biological pest control due to their broad host range. Entomopathogenic nematodes are effective and eco-friendly alternative to synthetic insecticides. Despite difficulties in mass production, formulation, and field conditions, their incorporation into pest control strategies bears significant promise. Advancements in biotechnology and formulation science can easily promote their applicability in sustainable agriculture. Recent research focuses on improving their field efficiency, genetic diversity, and commercialization. In some countries, EPNs are commercially produced for biological pest control in agriculture, horticulture, and forestry, however, efficient mass production and formulation are critical for their stability, storage, and field efficacy. EPNs are increasingly used in Africa for managing key agricultural pests due to their effectiveness against banana weevils, fall armyworm, sweet potato weevils, and white grubs, but the key challenges include, temperature sensitivity and farmer adoption, however, ongoing research and local production are improving accessibility. Recent studies in some African countries indicated appreciable success achieved with EPN use in Kenya (maize), Uganda (banana), Nigeria (sweet potato), and South Africa (maize grubs) and the key factors for the success include, proper application timing, farmer training, and local EPN adaptation among others.

KeyWords:

Entomopathogenic nematode, eco-friendly, bio-technology, stability and adoption

References:

  1. Abate, B. A., (2023). Biocontrol of banana weevil using Heterorhabditis indica in Uganda. Biological Control, 176, 105102. [DOI:10.1016/j.biocontrol.2023.105102]
  2. Ansari, M. A., Shah, F. A., & Butt, T. M. (2010). Biological Control, 53(3), 249-255.
  3. Atiri, G. J., (2023). Entomopathogenic nematodes for cassava root mealybug control in Ghana. Nematology, 25(3), 321-335. [DOI:10.1163/15685411-bja10232] 
  4. Bait F. C., Sorenson L., Santini F., and Alfaro M. E. (2013). A Phylogenomic perspective on the radiation of ray-finned fishes based upon targeted sequencing of ultra-conserved elements. PLOS ONE, 8(6)
  5. Campos-Herrera, R. (2023). Nematode Pathogenesis of Insects and Other Pests. Springer. [ISBN: 978-3-031-35287-2] 
  6. Chaston, J. M., Suen, G., Tucker, S. L., Andersen, A. W., Bhasin, A., Darby, C., & Goodrich-Blair, H. (2011). The entomopathogenic bacterial endosymbionts Xenorhabdus and Photorhabdus: convergent lifestyles from divergent genomes. PLoS ONE, 6(11), e27909.
  7. Dlamini, B. E., (2022). Control of white grubs in South African maize using Heterorhabditis zealandica. Journal of Applied Entomology, 146(5), 589-598. [DOI:10.1111/jen.13001] 
  8. Ekeh, F. N., (2021). Heterorhabditis bacteriophora for sweet potato weevil management in Nigeria. Crop Protection, 145, 105645. [DOI:10.1016/j.cropro.2021.105645] 
  9. Forst, S., & Clarke, D. (2002). Bacteria–nematode symbiosis. In: Gaugler R. (ed.). Entomopathogenic Nematology. CABI Publishing, pp. 57–77.
  10. Grewal PS (2002). 13 Formulation and Application Technology. Entomopathogenic Nematology P 265.
  11. Grewal, P. S., Ehlers, R. U., & Shapiro-Ilan, D. I. (2005). Nematodes as Biocontrol Agents. CABI.
  12. Grewal, P. S., Koppenhöfer, A. M., & Choo, H. Y. (1994). Insecticidal efficacy and persistence of Steinernema carpocapsae and Heterorhabditis bacteriophora in different soil types. Journal of Economic Entomology, 87(5), 1362–1371.
  13. John, M. G., Natalie, M. A. & Anjel, M.H. (2021). Chemical cues from entomopathogenic nematodes vary across three species with different foraging strategies, triggering different behavioural responses in prey and competitors. Journal of chemical ecology. Vol. 47, pp 822-833
  14. Kagimu, N., & Malan, A. P. (2024). Commercializing EPNs in Africa: Challenges and opportunities. Biocontrol, 69(1), 1-15. [DOI:10.1007/s10526-023-10232-3] 
  15. Kaya H. K., & Koppenhofer A. M. (1996). Effects of microbial and other antagonistic organisms and competition on EPNs. Biocontrol science and Technology, 6(3), 357-372
  16. Kaya, H. K., & Gaugler, R. (1993). Entomopathogenic nematodes. Annual Review of Entomology, 38, 181–206.
  17. Kaya, H. K., and Gaugler, R. (2022). Entomopathogenic Nematodes in Biological Control. Annual Review of Entomology, 67, 239-258. [DOI:10.1146/annurev-ento-060921-072718]
  18. Khashan H., Abu Ghazaleh F., Al Omari F., Al Essa N. Mahmoud N and Hassanein M. (2021). Primary healthcare reform in Saudi Arabia: progress, challenges and prospects. Eastern Mediteranian health journal, 27(10) 1016-1026
  19. Koppenhöfer AM (2007). Nematodes. In. Lacey LA, Kaya HK (eds), Field manual of techniques in invertebrate pathology: Application and evaluation of pathogens for control of insects and other invertebrate pests. Dordrecht, AA: Springer. pp. 249-264.
  20. Koppenhöfer, A. M., Shapiro-Ilan, D. I., & Grewal, P. S. (2020). Ecological characterization of entomopathogenic nematodes for commercial insect control. In: Nematode Pathogenesis of Insects and Other Pests, Springer, pp. 101–124.
  21. Lacey, L. A., & Georgis, R. (2012). Entomopathogenic nematodes for control of insect pests above and below ground with comments on commercial production. Journal of Nematology, 44(2), 218–225.
  22. Lewis EE, Clarke DJ (2012). Nematode parasites and entomopathogens. In. Vega FE, Kaya HK (eds), Insect pathology. Second ed. San Diego, CA: Academic Press pp. 395-443.
  23. Lulamba Tshikala Eddie, Ezekiel Green and Mahloro Hope Serepa-Dlamini (2019). Entomopathogenic nematodes, potential industrial pest control agents: A South African perspective. Journal of Entomology and Nematology. Vol. 11(1), pp. 1-12, January 2019 DOI: 10.5897/JEN2018.0210 Article Number: 243CF0B60140 ISSN 2006-9855
  24. Mwaniki, S. W. (2022). Field efficacy of Steinernema carpocapsae against fall armyworm in Kenya. Pest Management Science, 78(6), 2431-2438. [DOI:10.1002/ps.6874] 
  25. Pekar, S. (2021). Indirect effects of pesticides on insects and other arthropods. Toxics. 9(8), 177 
  26. Platt, T., N.F. Stokwe, A.P. Malan (2020). A Review of the Potential Use of Entomopathogenic Nematodes to Control Above-Ground Insect Pests in South Africa. S. Afr. J. Enol. Vitic., Vol. 41, No. 1, DOI: https://doi.org/10.21548/41-1-2424
  27. Poinar GO Jr (2011). Nematology Monographs and Perspectives. Volume 9. In. Hunt DJ, Perry RN (eds), The Evolutionary History of Nematodes. As Revealed in Stone, Amber and Mummies. Leiden Boston, MA: Brill pp. 1-5. 
  28. Shapiro-Ilan DI, Bruck DJ, Lacey LA (2012). Principles of epizootiology and microbial control. In. Vega FE, Kaya HK (eds), Insect pathology. Second ed. San Diego, CA: Academic Press. pp. 29-72.  
  29. Shapiro-Ilan, D. I. & Ehlers, R. U. (2023). Mass production of entomopathogenic nematodes in liquid culture. In: Nematodes as Biocontrol Agents (pp. 199-216). CABI. [DOI:10.1079/9781789242301.0012]
  30. Shapiro-Ilan, D. I. (2017). Journal of Nematology, 49(4), 373-382.
  31. Shapiro-Ilan, D. I., Gouge, D. H., & Koppenhöfer, A. M. (2001). Factors affecting commercial success: case studies in cotton, turf and citrus. In: Entomopathogenic Nematology, pp. 333–355.
  32. Stock, S. P., & Goodrich-Blair, H. (2012). Nematode parasites, pathogens and associates of insects and invertebrates of economic importance. In: Manual of Techniques in Invertebrate Pathology, 2nd ed., pp. 373–426.
  33. Tadesse, M. (2021). EPNs for wheat stem borer management in Ethiopia. Biocontrol, 66(4), 487-498. [DOI:10.1007/s10526-021-10094-7] 
  34. Waterfield N., Vlisidou I., Dowling A. J., Evans I. R., ffrench-Constant R. H. (2009). Drosophila embryos as model systems for monitoring bacterial infection in real time. PLOS Pathogens 5(7)
  35. Yasur, J., Glazer, I., & Sharon, E. (2020). Characterization of the phenotypic and Genotypic Tolerance to abiotic stresses in natural populations of EPNs. Scientific Reports, 10, 10500.