International Journal of Life Science and Agriculture Research

On-grown Artemia are ideally sized for feeding juveniles and adults of various popular ornamental fishes. With the simple technology, culture of Artemia can be easily implemented by ornamental fish hobbyists worldwide. Artemia live in hypersaline environment.  The use of crude salt dissolved in freshwater is a practical and easy alternative in making artificial culture media to obtain the desired salinity. In this study, we evaluated the effect of different salt levels of the culture media to produce on-grown Artemia in low salinity range from 15 – 65 ppt, trough observing their growth and survival. This study used a completely randomized experiment design. The Artemia nauplii were reared with 6 levels of media salinity, namely 15 ppt, 25 ppt, 35 ppt, 45 ppt, 55 ppt, and 65 ppt. Survival rate, length growth, and water quality parameters (salinity, temperature, pH, and dissolved oxygen) were observed on days 1, 10, and 21. Data were analyzed using one-way analysis of variance (ANOVA) determine differences between treatments. The results of the study showed that Artemia can grow optimally to adulthood at a salinity of 35 – 65 ppt.  Adult Artemia cultured for 21 days in a salinity of 35 – 65 ppt had an average body length of 7.99 ± 0.73 mm with an average survival rate of 47.39 ± 6.15%. We do not recommend rearing Artemia with too low salinities (15 and 25 ppt) because it results in poor Artemia growth and survival.

Brine shrimp, crude salt, low salinity, live food, ornamental fish, aquarium.

1. Agh, N., Van Stappen, G., Bossier, P., Sepehri, H., Lotfi, V., Razavi Rouhani, S.M., Sorgeloos, P. 2008. Effects of salinity on survival, growth, reproductive and life span characteristics of Artemia populations from Urmia Lake and Neighboring Lagoons.  Pak. J. Biol. Sci. 11(2) :164-172. 
2. Amin, M., Erwinda, M., Nissa, M., Nindarwi, D.D., Setyantini, W.H., Mubarak, A.S., Patmawati, Pujiastuti, D.Y.,  Sulmartiwi, L., Andriyono, S., Alamsjah, M.A.  2022. Fatty acids profiles and growth performances of Artemia franciscana fed with different types of microalgae. Sains Malaysiana 51(8): 2449-2459. https://doi.org/10.17576/jsm-2022-5108-09
3. Amin, M., Intan, B., Putri, M., Mukti, A.T., Alamsjah, M.A. 2023. Effect of protein sources in formulated diets on growth performance, feed utilization, survival rate, and reproductive performance of Artemia franciscana. Aquac. Int. 31(4): 1893-1910.   https://doi.org/10.1007/s10499-023-01059-x
4. Arce E., Archundia M.P.F., and Luna-Figueroa J. (2018). The effect of live food on the coloration and growth in Guppy Fish, Poecilia reticulata.  Agric. Sci. 9:171-179. https://doi.org/10.4236/as.2018.92013
5. Arumugam, P., Inbakandan, D., Ramasamy, M.S., Murugan, M. 2013. Encapsulated spirulina powder feed for the nutritional enrichment of adult Brine Shrimp (Artemia salina). J. Appl. Aquac., 25: 1–7, 2013.  

https://doi.org/10.1080/10454438.2013.817182

1. Castro-Mejía, J., Castro-Barrera, T., Hernández-Hernández, L.H., Arredondo-Figueroa, J.L., Castro-Mejía, G., Lara- Andrade, R.D. 2011. Effects of salinity on growth and survival in five Artemia franciscana (Anostraca: Artemiidae) populations from Mexico Pacific Coast. Int. J.  Trop. Biol. 59(1):199-206
2. Dhont, J., Diercken, K., Stottrup, J., Van Stappen, G., Wille, M., Sorgeloos, P., 2013. Rotifers, Artemia and Copepods as live feeds for fish larvae in aquaculture. In: Allan, G., Burnell, G., editors. Advances in Aquaculture Hatchery Technology. Oxford: Woodhead Publishing Limited. p. 157-202. https://doi.org/10.1533/9780857097460.1.157
3. Dhont, J., and Sorgeloos, P. 1996. Tank production and use of on-grown Artemia, In: Lavens, P., Sorgeloos, P. (Eds.) Manual on the production and use of live food for aquaculture. Rome: FAO Fisheries Technical Paper 361. P 164-195.
4. Islam, M.S., Arifuzzaman, M., Chowdhury, Hossain, M.B., Molla, M.H.R., Morshed, M.M., Bhuiyan, M.M.  2015. Production of brine shrimp, Artemia salina biomass and cyst in indoor tank using crude salt. Chem. Bio. Phy. Sci. Sec. B, 5(2): 1574-1584.
5. James, R. & Sampath, K. 2004.  Effect of feed type on growth and fertility in ornamental fish, Xiphophorus helleri. Bamidgeh 56(4) :264-273.
6. Khadka, N., Khadka, R., Mandal, R.B., Adhikari, A. 2023.  Growth performance of live fish feed: Artemia salina in different supplemental feeds in aquarium culture. J. Agric. Environ. 24:149-155.
7. Kiswara, C.A., Budiharjo, A., Sari, S.L.A. 2020. Changes in color of betta fish (Betta splendens) by feeding of Artemia salina enriched with Tagetes erecta flower flour. Cell. Biol. Dev. 4(2) :46-50. https://doi.org/10.13057/cellbioldev/v040202
8. Koru, E., & Turkmen, G.  2010. Recent developments on the application of artemia in the ornamental fish culture.  International Burch University Repository, Accessed July 9, 2024,   http://omeka.ibu.edu.ba/items/show/3090
9. Langroudi, H.E., Mousavi, S.H., Falahatkar, B., Moradkhani, Z.  2009.  Effect of diets containing Artemia enriched with unsaturated fatty acids and vitamin C on angel fish Pterophyllum scalare propagation. Int. Aquat. Res. 1:67-72.
10. Lavens, P. and Sorgeloos, P., 1991. Production of Artemia in culture tanks. In: Browne, R.A.; P.Sorgeloos and C.N.A. Trotman (Eds). Artemia biology. CRC press, Inc. Boca Ratón, Florida, USA., 317- 350.
11. Lim, L.C., Dhert, P., Sorgeloos, P. 2003. Recent developments in the application of live feeds in the freshwater ornamental fish culture. Aquaculture 227 (1-4) :319-331. 
12. Lim, L.C., Soh, A., Dhert, P., Sorgeloos, P. 2001. Production and application of on-grown Artemia in Freshwater ornamental fish. Aquac. Econ. Manag. 5 (3-4) :211- 228. https://doi.org/10.1080/13657300109380288
13. Lyubomirova, V., Romanova, E., Romanov, V., Fazilov, E., Shlenkina, T., Vasiliev, A., Sveshnikova, E. 2023. Features of Artemia salina ontogenesis in aquaculture depending on the salt level.  E3S Web of Conferences 381, 02022.  https://doi.org/10.1051/e3sconf/202338102022
14. Mohebbi, F., Hafezieh, M., Seidgar, M., Hosseinzadeh, S.H., Mohsenpour, A.A., Ahmadi, R. 2016. The growth, survival rate and reproductive characteristics of Artemia urmiana fed by Dunaliella tertiolecta, Tetraselmis suecica, Nannochloropsis oculata, Chaetoceros sp., Chlorella sp. and Spirulina sp. as feeding microalgae. Iranian J. Fish. Sci. 15(2) :727-737.
15. Ngarari, M.M., Hinzano, S.M., Opiyo, M.A., Rugendo, D.G., Midumbi, D.O., Okalo, F.A., Nyonje B.M., Ngugi, C.C., Gatune, C.W. 2024. Salinity tolerance, growth and survival of three Artemia franciscana (Kellogg, 1906) populations under laboratory conditions. Aquaculture, Fish and Fisheries, 4, e166. https://doi.org/10.1002/aff2.166
16. Noornissabegum, M., Madhavi, M., Mohanasundari, L. 2022. Influence of prey (Artemia nauplii) densities on the survival, growth and feed Consumption of Spotted Scat Scatophagus argus larvae, an indo-pacific Ornamental fish reared under controlled condition J. Adv. Sci. Res.13(1) :221-226. https://doi.org/10.55218/JASR.202213125
17. Saha, M.K. and Patra, B.C. 2013. Effect of growth and pigmentation on acceptability of different feeds by Colisa lalia (Hamilton, 1822).  J. Adv. Lab. Res. Biol.  4(3) :96-99.
18. Sales, J. & Janssens, G.P.J.  2003. Nutrient requirements of ornamental fish. Aquat. Living Resour. 16 :533–540
19. Seidgar, M., Mohebbi, F., Nekuiefard, A., Hafezieh, M., Dadgar, S., Anbi, A.A., et al. 2019. The effect of Artemia urmiana, Earthworm, Cow heart and concentrate as supplementary diets on skin color and pigmentation of Oscar fish (Astronotus ocellatus). Int. J. Aquat. Sci. 10(2) :88-93.
20. Sulistiyarto, B. & Restu. 2024. Survival, growth, and biomass of brine shrimp (Artemia franciscana) fed with spirulina powder and soybean flour.  Int. J. Fish. Aquat. Stud. 12(1) :13-18. https://doi.org/10.55677/ijlsar/V03I11Y2024-12
21. Suneetha, K., Padmavathi, P., & Chatla, D. (2024). Hatching and survival performance of Artemia franciscana under different salinities. Acta Biologica Turcica, 37(4), S8 :1-7.
22. Syukri F., Hassan N.H., Ani A.S.N. 2022. Performances of FS Feed, Artemia nauplii and commercial diet on early development of Clarias gariepinus larvae. J. Sustain. Sci. Mgmt. 17 (2) :35 – 45. http://doi.org/10.46754/jssm.2022.02.004
23. Vahdat, S., Oroujlou, M. 2021. Use of agriculture by-products (bran and meal) as food for Artemia franciscana (Kellogg, 1906) and effects on performance and biochemical compositions. J. Surv. Fish. Sci. 7(3) :23-40. 

http://dx.doi.org/10.18331/SFS2021.7.3.3

1. Van Stappen, G., 1996. Introduction, biology and ecology of Artemia. In: Lavens, P., Sorgeloos, P., editors. Manual on the production and use of live food for aquaculture. Rome: FAO Fisheries Technical Paper 361; p. 79-163. 
2. Wee, S., Loong, S., Ng, N.S.R., Cabana, F.  2021. Artemia as a sustainably cultured live feed for ornamental fish in zoological institutions with immunostimulant properties when bioencapsulated with spirulina Arthrospira platensis. J. Zoo. Aquar. Res. 9(2) :10-115. https://doi.org/10.19227/jzar.v9i2.546