Influence of Neem Leaves Powder in Litter Contamination, and Welfare Indicators of Broiler (Ross 308) Exposed to Heat Stress

Authors

  • Hashim Hadi Al-Jebory Agriculture college- Al-Qasim green university- Iraq
  • B. A. M. Lehmood Agriculture college- Al-Qasim green university- Iraq
  • Mohammed Khalil Ibrahim Al-Saeedi College of Environmental Sciences- Al-Qasim Green University- Iraq
  • Nihad Abdul-Lateef Ali Agriculture college- Al-Qasim green university- Iraq

DOI:

https://doi.org/10.55677/ijlsar/V02I12Y2023-04

Keywords:

Biosecurity, broiler welfare, HS, therapeutic alternatives.

Abstract

The increase in the emergence of the resistance of pathogenic bacteria against antibiotics in poultry farms increased the need to find therapeutic alternatives to raise the level of biosecurity, especially the high rate of disease infection during heat stress, which reduces broiler welfare. 320 chicks one-day old were used and were divided into four groups at random, each containing 80 chicks, Each group was made up of four replicates, and each group received a dose of 0, 200, 400, and 600 mg of neem leaf powder/ kg of diet for G1, G2, G3, and G4, respectively. All chicks were, exposed to (HS) (28-35-28 c⸰).  A significant heights (P≤0.05) for G2, G3, and G4 groups in benefits bacteria (Lactobacilli) of litter compared G1 group that increased significantly in E. coli bacteria and total bacteria. A significant heights (P≤0.05) in moisture and pH of litter and, feces weight for the G1 group compared to another group, in broiler welfare significant improvement (P≤0.05) in footpad dermatitis and feather hygiene percentage for G2, G3, and G4 groups compared G1 group. These results conclude that the neem leaf powder added to the broiler feed increased the number of beneficial bacteria and reduced the number of harmful bacteria, as well as improved the physical characteristics of the litter, which achieved positive results in the welfare of broilers raised under conditions of heat stress.

References

Abdelqader, A.; Al-Fataftah, A.-R. Effect of dietary butyric acid on performance, intestinal morphology, microflora composition and intestinal recovery of heat-stressed broilers. Livest. Sci. 2016, 183, 78–83.

Agboola, A. F.; Omidiwura, B. R. O.; Odu, O.; Odupitan, F. T. and Iyayi, E. A. (2015). Effect of probiotic and toxin binder on performance, intestinal microbiota and gut morphology in broiler chickens. J. Anim. Sci. Adv., 5(7): 1369-1379.

ALakomi, H.L., E. Skytta, A. Saarela, M. Mattila-sandholm, T. Latv, K. A-Kala, and I.M. Helander. 2000. Lactic Acid Penneabilizes Gram-Negative Bacteria by Disrupting the Outer Membrane. Applied and Environmental Microbiology .66(5): 2001-2005.

Al-Jebory, H.H. and S.A.H. Naji. 2021 a. Effect of Pelleted Fermented Feed in Production Performance of Laying Hens. Fourth International Conference for Agricultural and Sustainability Sciences IOP Conf. Series: Earth and Environmental Science 910 (2021) 012007 IOP Publishing doi:10.1088/1755-1315/910/1/012007.

Al-Jebory, H.H., M. K. I. Al-Saeedi., I. L. Al-Jaryan., and F.R.Al-Khfaji.,2023. Impact of Neem (Azadirachta Indica) leaves powder on growth performance of broiler (Ross 308) exposed to HS. Research Journal of Agriculture and Biological Sciences, 15(2): 1-5. DOI: 10.22587/rjabs.2023.15.2.1

Aljebory, H.H.D S.A.H. Naji. 2021 b. Effect of Pelleted Fermented Feed-in Egg Quality of Laying Hens. Diyala Agricultural Sciences Journal 13 (1): 41-57. https://dx.doi.org/10.52951/dasj.21130105.

Alnajjar, E. and Alemadi, M.A. (2017). Evaluation of the impact of some disinfectants used in poultry farms. Assiut Veterinary Medical Journal., 63 (152): 1-7.

Al-Salhi, A.A., S.M. Al-Shatty, and E.A. AlImar. 2022. Effect of Manufactured Bacterial Preparation in Reducing Rates of Contamination Resulting from Laying Hens. Syrian Journal of Agricultural Research –SJAR. 9(4): 154-164.

Beal, R.K., C. Powers, T.F. Davison, and A.L. Smith. 2006. Immunological Development of the avian gut. in Avian Gut Function i11 Health and Disease (Ed G. C. Perry): 85-103. Wallingford, U. K.: CAB International.

Bonsu FRK, Kagya-Agyemang JK, Kwenin WKJ and Zanu HK. Medicinal response of broiler chickens to diets containing neem (Azadirachta indica) leaf meal, haematology and meat sensory analysis. World Applied Sciences Journal, 19: 800- 805. 2012.

Borges, A., C. Ferreira, M.J. Saavedra, and M. Simoes. 2013. Antibacterial Activity and Mode of Action of Ferulic and Gallic Acids Against Pathogenic Bacteria. MICROBIAL DRUG RESISTANCE. DOI: 10.1089/mdr.2012.0244.

Burkholder, K.M., K.L. Thompson, M.E. Einstein, T.J. Applegate, and J.A. Patterson. 2008. Influence of stressors on normal intestinal microbiota, intestinal morphology, and susceptibility to Salmonella enteritidis colonization in broilers. Poult. Sci. 87, 1734–1741. https://doi.org/10.3382/ps.2008-00107.

Correa, T.A.F.; Rogero, M.M.; Hassimotto, N.M.A.; Lajolo, F.M. The Two-Way Polyphenols-Microbiota Interactions and Their Effects on Obesity and Related Metabolic Diseases. Front. Nutr. 2019, 6.

Del Vesco, A.P., E. Gasparino, D.O. Grieser, V. Zancanela, M.A.M. Soares, and A.R. Oliveira Neto. 2015. Effects of methionine supplementation on the expression of oxidative stress-related genes in acute HS-exposed broilers. The British Journal of Nutrition 113, 549–559.

Del Vesco, A.P., E. Gasparino, V. Zancanela, D.O. Grieser, C.E. Stanquevis, P.C. Pozza, and A.R. Oliveira Neto. 2017. Effects of selenium supplementation on the oxidative state of acute HS-exposed quails. Journal of Animal Physiology and Animal Nutrition 101, 170–179.

Deng, X., Z. Li, and W. Zhang. 2012. Transcriptome sequencing of Salmonella enterica serovar Enteritidis under desiccation and starvation stress in peanut oil. Food Microbiol. 30, 311–315. https://doi.org/10.1016/j.fm.2011.11.001.

Duncan, D.B. 1955. Multiple Rang and Multiple F-test. Biometrics. 11: 4-42.

Espin, J.C.; Gonzalez-Sarrias, A.; Tomas-Barberan, F.A. The gut microbiota: A key factor in the therapeutic effects of (poly)phenols. Biochem. Pharmacol. 2017, 139, 82–93.

Ezzat, H.N., S.S Abood, and H.S.A. Jawad. 2018. A review on the effects of neem (Azadirachta indica) as feed additive in poultry production. Journal of Entomology and Zoology Studies. 6(1): 1331-1333.

Garces, A. P. J. T., Afonso, S. M. S., Chilundo, A., and Jairoce, C. T. S. (2013). Evaluation of different litter materials for broiler production in a hot and humid environment: 1. Litter characteristics and quality. Journal of Applied Poultry Research, 22(2), 168-176.

Gasparino, E., A.P. Del Vesco, A.S. Khatlab, V. Zancanela, D.O. Grieser, S.C.C. Silva. 2018. Effects of methionine hydroxy analogue supplementation on the expression of antioxidant-related genes of acute HS-exposed broilers. Animal 12, 931–939.

GONDER, E. and BARNES, H.J. (1987) Focal ulcerative dermatitis (breast buttons) in market turkeys. Avian Diseases 31(1): 52-58.

Heres, L., B. Engel, H.A.P. Urlings, J.A. Wagenaar, and F.V. Knapen. 2004. Effect of acidified feed on susceptibility of broiler chickens to intestinal infection by Campylobacfer and Salmonella. Veterinm:v Microbiology. 99(3-4): 259- 267.

Heyman L, Houri-Haddad Y, Heyman SN, Ginsburg I, Gleitman Y and Feuerstein O. Combined antioxidant effects of Neem extract, bacteria, red blood cells and Lysozyme: possible relation to periodontal disease. BMC Complementary and Alternative Medicine, 17: 399. 2017.

Hoskins , B. , A. Wolf, and N. Wolf. 2003. Dry matter analysis. Recommended methods of manure analysis (A3769). I-2/2003. (online) Available at: http://cecommerce.uwex.edu/pdfs/A3769.pdf.

Hwang, H. and Singer, R. S. (2020). Survey of the US broiler industry regarding pre-and postharvest interventions targeted to mitigate Campylobacter contamination on broiler chicken products. Journal of Food Protection, 83(7): 1137-1148.

KAMYAB, A. (2001) Enlarged sternal bursa and focal ulcerative dermatitis in male turkeys. World’s Poultry Science Journal 57: 5-12.

Karcher, D. M., Makagon, M. M., Fraley, G. S., Fraley, S. M., and Lilburn, M. S. (2013). Influence of raised plastic floors compared with pine shaving litter on environment and Pekin duck condition. Poultry science, 92(3), 583-590.

Kaukonen, E., Norring, M., and Valros, A. (2016). Effect of litter quality on foot pad dermatitis, hock burns and breast blisters in broiler breeders during the production period. Avian pathology, 45(6), 667-673.

Lara, L.J., and M.H. Rostagno. 2013. Impact of HS on poultry production. Animals 3, 356–369.

Lillehoj, H.; Liu, Y.; Calsamiglia, S.; Fernandez-Miyakawa, M.E.; Chi, F.; Cravens, R.L.; Oh, S.; Gay, C.G. Phytochemicals as antibiotic alternatives to promote growth and enhance host health. Vet. Res. 2018, 49, 76

Makeri H.K., Maikai V.A. & Nok J.A. (2007) Effect of topical application of neem seed (Azadirachta indica) extract on sheep infested with Amblyomma variegatum. African Journal of Biotechnology 6, 2324–2327.

Mayne, R.K. 2005. A review of the aetiology and possible causative factors of foot pad dermatitis in growing turkeys and broilers. World’s Poultry Science Journal. 61. 256-267. DOI: 10.1079/WPS200458.

Moran, C.A., R.H.J. Scholten, J.M. Tricarico, P.H. Brooks, and M.W.A. Verstegen. 2006. Fomentation of wheat: Effects of backslopping different propo1ions of pre-fermented wheat on the microbialand chemical composition. Archives of Animal Nutrition. 60(2): 158-169.

Nnenna OP and Okey AA. Toxicity and nutritional assessment of aqueous Azadirachta indica (neem) leaf extract in broiler chicks. International Journal of Biosciences, 3: 172-180. 2013.

Saleem S, Muhammad G, Hussain MA and Bukhari SNA. A comprehensive review of phytochemical profile, bioactives for pharmaceuticals, and pharmacological attributes of Azadirachta indica. Phytotherapy Research, 32: 1241-1272. 2018.

Samy, R.P., and P. Gopalakrishnakone. 2010. Therapeutic potential of plants as anti-microbials for drug discovery. Evid. Based Complement. Alternat. Med. 7:283–294.

SAS, 2012. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.

Shewale S and Rathod VK. Extraction of total phenolic content from Azadirachta indica or (neem) leaves: Kinetics study. Prep Biochem Biotechnol, 48: 312-320. 2018.

Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., & Crocker, D. L. A. P. (2008). Determination of structural carbohydrates and lignin in biomass. Laboratory analytical procedure, 1617(1), 1-16.

Sonhafouo, V.M., J.R. Kana, and K.N. Dongmo. 2019. Effects of graded levels of Azadirachta indica seed oil on growth performance and biochemical profiles of broiler chickens. Veterinary Medicine and Science. 5: 442–450. DOI: 10.1002/vms3.162.

Stalikas, C.D. 2007. Extraction, separation, and detection methods for phenolic acids and flavonoids. J. Sep. Sci. 30:3268–3295.

Theilmann, M.C.; Goh, Y.J.; Nielsen, K.F.; Klaenhammer, T.R.; Barrangou, R.; Abou Hachem, M. Lactobacillus acidophilus metabolizes dietary plant glucosides and externalizes their bioactive phytochemicals. MBio 2017, 8.

Valarmathy K, Gokulakrishnan M, Kausar MS, Paul K. A study of antimicrobial activity of ethanolic extracts of various plant leaves against selected microbial species. International Journal of Pharma Sciences and Research, 2010; 1(8):293-295.

Valarmathy K., Gokulakrishnan M., Kausar M.S. & Paul K.A. (2010) Study of antimicrobial activity of ethanolic extracts of various plant leaves against selected microbial species. International Journal of Pharmacological Sciences and Research 1, 293–295.

Viveros, A.; Chamorro, S.; Pizarro, M.; Arija, I.; Centeno, C.; Brenes, A. Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Poult. Sci. 2011, 90, 566–578.

Wang, B.-N., H.F. Liu, J.B. Zheng, M.T. Fan, and W. Cao. 2011. Distribution of phenolic acids in different tissues of Jujube and their antioxidant activity. J. Agric. Food Chem. 59:1288–1292.

Xing, S.; Wang, X.; Diao, H.; Zhang, M.; Zhou, Y.; Feng, J. Changes in the cecal microbiota of laying hens during HS is mainly associated with reduced feed intake. Poult. Sci. 2019, 98, 5257–5264.

Downloads

Published

2023-12-16

How to Cite

Hashim Hadi Al-Jebory, B. A. M. Lehmood, Mohammed Khalil Ibrahim Al-Saeedi, & Nihad Abdul-Lateef Ali. (2023). Influence of Neem Leaves Powder in Litter Contamination, and Welfare Indicators of Broiler (Ross 308) Exposed to Heat Stress. International Journal of Life Science and Agriculture Research, 2(12), 497–504. https://doi.org/10.55677/ijlsar/V02I12Y2023-04